Localization of mitochondrial DNA in normal and pathological muscle using immunological probes: a new approach to the study of mitochondrial myopathies.

Abstract

We have studied the usefulness of anti-DNA antibodies to detect ragged-red fibers (RRF) in muscle biopsies from patients with mitochondrial myopathies. We have found that these antibodies are excellent probes for the localization of mitochondrial DNA (mtDNA) in RRF, and for the diagnosis of depletion of mtDNA in a newly described group of fatal myopathies of infancy.

• Authors: F Andreetta, H J Tritschler, E A Schon, S DiMauro, E Bonilla
• Journal: Journal of the neurological sciences (J Neurol Sci) Vol. 105 Issue 1 Pg. 88-92 (Sep 1991) ISSN: 0022-510X [Print] Netherlands
• PMID: 1665507 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies
• Antibodies, Monoclonal
• DNA, Mitochondrial
• Succinate Dehydrogenase
• Electron Transport Complex IV

Topics

• Antibodies
• Antibodies, Monoclonal
• Biopsy
• DNA, Mitochondrial (analysis, genetics)
• Electron Transport Complex IV (analysis)
• Humans
• Immunohistochemistry
• Kearns-Sayre Syndrome (genetics, pathology)
• Mitochondria, Muscle (enzymology, ultrastructure)
• Muscles (enzymology, pathology)
• Muscular Diseases (genetics, pathology)
• Ophthalmoplegia (genetics, pathology)
• Reference Values
• Succinate Dehydrogenase (analysis)