Granulosa-
theca cell tumors (GTCTs) are able to secrete variable amounts of sex
steroids and immunoreactive
inhibin (ir-INH). Although the pituitary appears to be affected by the presence of a GTCT, pituitary responsiveness to exogenous
GnRH has not been examined. The aims of the present study were to: (i) assess the plasma
hormone concentrations of ir-INH,
gonadotropins and sex
steroids in eight mares with GTCT and (ii) assess the responsiveness of pituitary gonadotroph cells to exogenous
GnRH stimulus both before and after
tumor removal. In seven mares, the contralateral ovary was firm, small and inactive. Histopathological observations of the
tumors confirmed the presumptive diagnosis of a GTCT. Four mares, judged to be in vernal transition period (n=2) and in the breeding season (n=2), were used as controls. A single
intravenous injection of 40 microg of
GnRH agonist was given to each mare and blood samples were collected every 15 min from 2 h before to 4 h after injection. In four GTCT mares, this procedure was repeated 20 (n=2) and 90 (n=2) days after
tumors removal. All plasma samples were analyzed for concentrations of ir-INH, LH, FSH,
estradiol-17beta (E2),
testosterone (T) by RIA and
progesterone (P) by EIA. Results showed that E2 levels were significantly higher (P<0.001) in control animals compared to E2 levels in GTCT mares before and after surgery. P and T concentrations were not statistically different between the groups. Baseline levels of ir-INH were greater (P<0.05) in GTCT mares before surgery than in control mares, and decreased to undetectable levels after
neoplasia ablation. Baseline FSH did not differ between control and GTCT animals either before or after the ovaries were removed. LH baseline values appeared to be higher for affected mares, but the difference was not statistically significant. Maximum release (MR) and area under the gonadotrophin release curve (AUC) after the
GnRH challenge for both the gonadotrophins were similar between the groups.