Preventive effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate derived from wasabi (Wasabia japonica Matsum) against pulmonary metastasis of B16-BL6 mouse melanoma cells.

Abstract

AIM:

Effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) or a 6-MITC-containing T-wasabi fraction from wasabi root (Wasabia japonica Matsum) to inhibit the macroscopic pulmonary metastasis was studied with a murine B16-BL6 melanoma model.

METHOD:

Two administration routes, subcutaneous or intravenous, and two administration times, prior to or concomitant with tumor inoculation, of 6-MITC or T-wasabi against the metastatic foci formation in C57BL/6J mouse lungs were compared.

RESULTS:

The number of metastasized foci per lung in either subcutaneous or intravenous injection was significantly reduced by intake of 6-MITC or a T-wasabi fraction. The maximum reduction by a T-wasabi fraction reached to 82%. Fifty-six percent of foci formation was inhibited by a 2 week-prior administration of 6-MITC (200 microM), whereas only 27% inhibition was obtained by a concomitant administration with tumor inoculation. Neither 6-MITC nor T-wasabi at tested concentrations showed any toxic effects.

DISCUSSION:

Together with our previous results, a component of the Japanese pungent spice, wasabi appears to inhibit not only tumor cell growth but also tumor metastasis. Therefore, 6-MITC from wasabi is apparently a useful dietary candidate for controlling tumor progression.

• Authors: Yoko Fuke, Shoko Shinoda, Ikuko Nagata, Saeko Sawaki, Mituyoshi Murata, Kazuo Ryoyama, Keiichi Koizumi, Ikuo Saiki, Takahiro Nomura
• Journal: Cancer detection and prevention (Cancer Detect Prev) Vol. 30 Issue 2 Pg. 174-9 ( 2006) ISSN: 0361-090X [Print] England
• PMID: 16647224 (Publication Type: Journal Article)

Chemical References

• 6-(Methylsulfinyl)hexyl isothiocyanate
• Antineoplastic Agents
• Isothiocyanates
• Plant Preparations

Topics

• Administration, Oral
• Animals
• Antineoplastic Agents (administration & dosage, pharmacology)
• Cell Line, Tumor (drug effects)
• Cell Proliferation (drug effects)
• Isothiocyanates (administration & dosage, chemical synthesis, pharmacology)
• Lung Neoplasms (drug therapy, prevention & control, secondary)
• Male
• Melanoma, Experimental (drug therapy, prevention & control, secondary)
• Mice
• Mice, Inbred C57BL
• Phytotherapy
• Plant Preparations (pharmacology)
• Plant Roots
• Wasabia
• Xenograft Model Antitumor Assays