[The use of lanthanide immunofluorescence analysis for demonstrating the antigen of viral hepatitis A].

Abstract

The study dealt with the development of an express method for detection of hepatitis A virus (HAV) antigen employing lantanides, especially europium. The new time-resolved fluoroimmunoassay (TRFIA), alongside with a significant shortening of the time for reaction, was also 8 times as sensitive as the analogous enzyme immunoassay. The TRFIA may be used effectively both for control of the antigen amplification in HAV-infected cell culture and for early diagnosis of acute forms of viral hepatitis A.

• Authors: M A Chernovetskiĭ, M V Sokolova, I G Kharitonenkov
• Journal: Voprosy virusologii (Vopr Virusol) 1991 Nov-Dec Vol. 36 Issue 6 Pg. 450-2 ISSN: 0507-4088 [Print] Russia (Federation)
• Vernacular Title: Primenenie lantanidnogo immunofliuorestsentnogo analiza dlia indikatsii antigena virusnogo gepatita A.
• PMID: 1664554 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Antigens, Viral
• Metals, Rare Earth

Topics

• Antigens, Viral (blood)
• Evaluation Studies as Topic
• Fluoroimmunoassay (methods)
• Hepatovirus (immunology)
• Humans
• Immunoenzyme Techniques
• Metals, Rare Earth