Antiviral activity of the prostanoid clavulone II against vesicular stomatitis virus.

Abstract

Prostaglandins of the A series exhibit the most pronounced antiviral activity in cells infected with RNA or DNA viruses as compared to other prostaglandins. Clavulone is a prostaglandin A analog found in the soft coral Clavularia viridis. Using vesicular stomatitis virus in mouse L929 fibroblasts as a model system, 50% inhibition of viral yield was seen at a concentration of 1-1.5 microM, whereas 50% cytotoxicity required 50-70 times higher inhibitor concentrations. For a further elucidation of the antiviral mechanism a temperature-sensitive mutant, tsG 41, was used, which is replication-negative at the restrictive temperature. Results obtained with this mutant suggest that inhibition of VSV replication occurs at the level of transcription.

• Authors: T Bader, Y Yamada, H Ankel
• Journal: Antiviral research (Antiviral Res) Vol. 16 Issue 4 Pg. 341-55 (Dec 1991) ISSN: 0166-3542 [Print] Netherlands
• PMID: 1663734 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antiviral Agents
• Prostaglandins A
• RNA, Messenger
• RNA, Viral
• Viral Proteins
• clavulone II
• prostaglandin A1

Topics

• Animals
• Antiviral Agents (pharmacology)
• Blotting, Northern
• Blotting, Western
• Cell Death (drug effects)
• In Vitro Techniques
• L Cells (drug effects)
• Mice
• Molecular Structure
• Prostaglandins A (chemistry, pharmacology)
• Protein Biosynthesis (drug effects)
• RNA, Messenger (genetics)
• RNA, Viral (analysis, genetics)
• Vesicular stomatitis Indiana virus (drug effects)
• Viral Plaque Assay
• Viral Proteins (metabolism)
• Virus Replication (drug effects)