Isolation of equine herpesvirus-1 mutants in the presence of (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine: demonstration of resistance in vitro and in vivo.

Abstract

The compound (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA) had been previously shown to be highly effective in treatment of EHV-1 in a murine model for the equine disease. This paper describes the isolation of a series of mutants resistant to the drug. Resistance was demonstrated in cell culture and one mutant was tested in a murine model. The resistant mutant was pathogenic for mice; infectious virus was recovered from respiratory tissues and blood at levels similar to the parental virus. However, the mutant showed a significant degree of resistance in vivo, thus proving the virus-specific mode of action of the antiviral compound.

• Authors: H J Field, A R Awan, R de la Fuente
• Journal: Antiviral research (Antiviral Res) Vol. 16 Issue 1 Pg. 29-39 (Jul 1991) ISSN: 0166-3542 [Print] Netherlands
• PMID: 1663728 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Organophosphonates
• Organophosphorus Compounds
• 9-(S)-(3-hydroxy-2-(phosphonomethoxy)propyl)adenine
• Adenine

Topics

• Adenine (analogs & derivatives, pharmacology, therapeutic use)
• Animals
• Antiviral Agents (pharmacology, therapeutic use)
• Cell Line
• Drug Resistance, Microbial
• Female
• Herpesviridae Infections (drug therapy, microbiology)
• Herpesvirus 1, Equid (drug effects, genetics, isolation & purification)
• Mice
• Mice, Inbred BALB C
• Mutation
• Organophosphonates
• Organophosphorus Compounds (pharmacology, therapeutic use)
• Phenotype