Abstract |
We used immunohistochemistry and flow cytometry to assess apoptosis in human glioblastoma multiforme (GBM). Our immunohistochemical study revealed apoptosis of glioma cells expressing glial fibrillary acidic protein and of CD3(+) T cells infiltrating GBM. To quantify and phenotype the apoptotic T cells, we performed flow cytometry on lymphocytes separated from GBM. The cells were stained with annexin-V- FLUOS/ propidium iodide to identify apoptosis. We found that high proportions of both the CD4(+) and CD8(+) T cells were apoptotic. In particular, we found that T cells expressing Fas ligand (Fas-L, CD95L) were eight times more vulnerable to apoptosis than those not expressing Fas-L, which suggests that the T-cell apoptosis is induced by overactivation of the T-cell receptor, possibly in the absence of appropriate costimulation. Our results have implications for the design of immunotherapies for GBM.
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Authors | David G Walker, Teong Chuah, Michael J Rist, Michael P Pender |
Journal | Journal of neuroimmunology
(J Neuroimmunol)
Vol. 175
Issue 1-2
Pg. 59-68
(Jun 2006)
ISSN: 0165-5728 [Print] Netherlands |
PMID | 16631933
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Apoptosis
(immunology)
- Flow Cytometry
- Glioblastoma
(immunology, pathology, therapy)
- Humans
- Immunohistochemistry
- Immunotherapy
- In Situ Nick-End Labeling
- Lymphocytes, Tumor-Infiltrating
(immunology, pathology)
- T-Lymphocytes
(immunology, pathology)
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