Abstract | BACKGROUND: OBJECTIVE: To explore the localization and biological role of ATP2C1 in normal epidermis in terms of differentiation states. METHODS: We examined the immunohistochemical distribution of ATP2C1 in normal epidermis and measured the expression of ATP2C1 in cultured keratinocytes following forced detachment from culture dish or following treatment with high concentrations of calcium. Furthermore, we knockdown ATP2C1 expression in cultured keratinocytes by using RNA interference procedure to abrogate cation accumulation in cell organelles. RESULTS: CONCLUSION: Our results suggest that ATP2C1 plays an essential role for basal keratinocytes to keep in the undifferentiated state and that its reduction evokes differentiation and up-localization to suprabasal layers most likely via the manganese starvation in the Golgi apparatus of keratinocytes.
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Authors | Masaki Yoshida, Kazuo Yamasaki, Takashi Daiho, Hajime Iizuka, Hiroshi Suzuki |
Journal | Journal of dermatological science
(J Dermatol Sci)
Vol. 43
Issue 1
Pg. 21-33
(Jul 2006)
ISSN: 0923-1811 [Print] Netherlands |
PMID | 16621454
(Publication Type: Journal Article)
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Chemical References |
- Ionophores
- Calcimycin
- Manganese
- 4-bromo-A-23187
- DNA
- ATP2C1 protein, human
- Calcium-Transporting ATPases
- Calcium
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Topics |
- Base Sequence
- Calcimycin
(analogs & derivatives, pharmacology)
- Calcium
(pharmacology)
- Calcium-Transporting ATPases
(genetics, metabolism)
- Cell Adhesion
- Cell Differentiation
(drug effects, physiology)
- Cells, Cultured
- DNA
(genetics)
- Epidermal Cells
- Epidermis
(enzymology)
- Golgi Apparatus
(metabolism)
- Humans
- Ionophores
(pharmacology)
- Keratinocytes
(cytology, drug effects, enzymology)
- Manganese
(metabolism)
- Models, Biological
- RNA Interference
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