Nanoparticles of cationic amphiphilic cyclodextrins entangling anionic porphyrins as carrier-sensitizer system in photodynamic cancer therapy.

Abstract

The photodynamic activity of a carrier-sensitizer system consisting of heterotopic colloidal nanoparticles (diameter 100-1000 nm) of a cationic amphiphilic cyclodextrin, heptakis(2-omega-amino-O-oligo(ethylene oxide)-6-hexylthio)-beta-CD (SC6CDNH2) encapsulating the anionic 5,10,15,20-tetrakis(4-sulfonatophenyl)-21H,23H-porphyrin (TPPS) is investigated by an interdisciplinary approach involving the combination of time-resolved absorption and emission techniques with in vitro studies on cultured tumor cells. In a range of TPPS:SC6CDNH2 molar ratios between 1:10 and 1:50 these nanoparticles preserve the photodynamic properties of the entrapped photoactive agent. In fact, the triplet state of TPPS is efficiently populated, very long-lived and, as a consequence, able to produce singlet oxygen (the essential species for the photodynamic action) with quantum yield comparable to the free TPPS. Photodynamic efficacy of the carrier/sensitizer system is proven by in vitro studies on tumor Hela cells treated with TPPS:SC6CDNH2 at different molar ratio, showing significant cells death upon illumination with visible light.

• Authors: Salvatore Sortino, Antonino Mazzaglia, Luigi Monsù Scolaro, Francesca Marino Merlo, Vincenza Valveri, Maria Teresa Sciortino
• Journal: Biomaterials (Biomaterials) Vol. 27 Issue 23 Pg. 4256-65 (Aug 2006) ISSN: 0142-9612 [Print] Netherlands
• PMID: 16620960 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclodextrins
• Porphyrins
• Surface-Active Agents

Topics

• Cyclodextrins
• Drug Delivery Systems
• HeLa Cells
• Humans
• Nanostructures
• Neoplasms (drug therapy)
• Photochemotherapy
• Porphyrins
• Surface-Active Agents