Abstract | OBJECTIVE: METHODS: T cells were co-cultured with (60)Co-irradiated bone marrow MSCs from different individuals. The proliferative activity of T cells and their reactivity to allogeneic cells and ConA were evaluated with (3)H-TdR incorporation assay. RESULTS: T cells could not be activated upon primary or even secondary exposure to allogeneic MSCs (compared the CPM value of 27,529 +/- 969 of T cell alone with that of primary and secondary exposures to allogeneic MSCs were 9,126 +/- 654 and 13,260 +/- 874, respectively). When MSCs were induced to express HLA-DR, they still could not elicit T cell activation. The proliferation rate of allogenous T cells exposed to MSCs was dramatically declined when T cells from the same donor's MSCs were used as stimulator (CPM value decreased from 45,876 +/- 5285 before coculture to 9850 +/- 1618 after coculture). Furthermore, the results remained unchanged even ConA was added into the culture system. CONCLUSIONS: Heterogenetic MSCs could suppress T cell activation. MSCs pretreatment might be useful in the prevention of GVHD in HLA-mismatched bone marrow transplantation.
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Authors | Jian-lin Chen, Kai Feng, Zi-Kuan Guo, Ren-na U, Chen Xu, Yu-hang Li, Xiao-dan Liu, Ning Mao, Hu Chen |
Journal | Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
(Zhonghua Xue Ye Xue Za Zhi)
Vol. 26
Issue 12
Pg. 740-2
(Dec 2005)
ISSN: 0253-2727 [Print] China |
PMID | 16620579
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Bone Marrow Cells
(immunology)
- Cell Communication
(immunology)
- Cell Proliferation
- Cells, Cultured
- Coculture Techniques
- Graft vs Host Disease
(immunology, prevention & control)
- Humans
- Lymphocyte Activation
- Mesenchymal Stem Cells
(immunology)
- T-Lymphocytes
(immunology)
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