Abstract | PURPOSE OF INVESTIGATION: METHODS: For the experiments human ovarian cancer cells (OVCAR-3) were used. The progestogens were tested at the concentrations of 0.01 to 10 microM. The growth factor mixture consisted of EGF, FGF and IGF-I, each at a concentration of 10 pM. The incubation time was three or seven days. Proliferation rate was measured by an ATP-assay. RESULTS: After three days' incubation the growth factors induced an increase in the proliferation rate of about 50%. Progesterone alone did not show any significant change as compared to the control values, whereas NET and MPA elicited a significant increase at 1 and 10 microM and at 1 microM, respectively. In the presence of growth factors none of the progestogens was able to inhibit the proliferative stimulation. After seven days' incubation the growth factors still showed an increase of about 50%. MPA alone had an inhibitory effect at 10 microM, for NET and P no effects were observed. Again in the presence of growth factors no progestogen was able to show an inhibitory effect. CONCLUSION: Our results indicate that progestogens do not have a protective role on the growth of pre-existing ovarian cancer cells, at least in the presence of growth factors. Further investigations are worthwile to evaluate possible differences between the effect of the various progestogens.
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Authors | H Seeger, D Wallwiener, A O Mueck |
Journal | European journal of gynaecological oncology
(Eur J Gynaecol Oncol)
Vol. 27
Issue 2
Pg. 139-41
( 2006)
ISSN: 0392-2936 [Print] Singapore |
PMID | 16620055
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents, Hormonal
- Insulin-Like Growth Factor Binding Proteins
- Progestins
- Protective Agents
- Fibroblast Growth Factors
- Epidermal Growth Factor
- Medroxyprogesterone Acetate
- Norethindrone
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Topics |
- Analysis of Variance
- Antineoplastic Agents, Hormonal
(pharmacology)
- Cell Line, Tumor
(drug effects)
- Cell Proliferation
(drug effects)
- Epidermal Growth Factor
(pharmacology)
- Female
- Fibroblast Growth Factors
(pharmacology)
- Humans
- In Vitro Techniques
- Insulin-Like Growth Factor Binding Proteins
(pharmacology)
- Medroxyprogesterone Acetate
(pharmacology)
- Norethindrone
(pharmacology)
- Ovarian Neoplasms
(drug therapy, metabolism)
- Progestins
(pharmacology)
- Protective Agents
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