Abstract | BACKGROUND & AIMS: METHODS:
Bile acid composition was analyzed by mass spectroscopy. Body weight, food intake, energy expenditure, and fat absorption were determined in animals fed either a low- or a high-fat diet. RESULTS: Although total bile acid concentrations were unchanged in bile, liver, urine, and feces of FATP5 knockout mice, the majority of gallbladder bile acids was unconjugated, and only a small percentage was conjugated. Primary, but not secondary, bile acids were detected among the remaining conjugated forms in FATP5 deletion mice, suggesting a specific requirement for FATP5 in reconjugation of bile acids during the enterohepatic recirculation. Fat absorption in FATP5 deletion mice was largely normal, and only a small increase in fecal fat was observed on a high-fat diet. Despite normal fat absorption, FATP5 deletion mice failed to gain weight on a high-fat diet because of both decreased food intake and increased energy expenditure. CONCLUSIONS: Our findings reveal an important role for FATP5 in bile acid conjugation in vivo and an unexpected function in body weight homeostasis, which will require further analysis. FATP5 deletion mice provide a new model to study the intersection of bile acid metabolism, lipid metabolism, and body weight regulation.
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Authors | Brian Hubbard, Holger Doege, Sandhya Punreddy, Hui Wu, Xueming Huang, Virendar K Kaushik, Robin L Mozell, John J Byrnes, Alain Stricker-Krongrad, Chieh J Chou, Louis A Tartaglia, Harvey F Lodish, Andreas Stahl, Ruth E Gimeno |
Journal | Gastroenterology
(Gastroenterology)
Vol. 130
Issue 4
Pg. 1259-69
(Apr 2006)
ISSN: 0016-5085 [Print] United States |
PMID | 16618417
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Bile Acids and Salts
- Dietary Fats
- Fatty Acid Transport Proteins
- Slc27a5 protein, mouse
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Topics |
- Absorption
- Aging
(metabolism)
- Animals
- Bile Acids and Salts
(metabolism)
- Body Weight
- Dietary Fats
(administration & dosage)
- Dose-Response Relationship, Drug
- Eating
- Energy Metabolism
- Fatty Acid Transport Proteins
(deficiency)
- Gallbladder
(metabolism)
- Gene Expression
- Lipid Metabolism
- Male
- Mice
- Mice, Knockout
- Obesity
(etiology, prevention & control)
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