Determination of desoxyepothilone B in nude mice plasma by liquid-liquid extraction and reversed-phase high-performance liquid chromatography.

Abstract

A novel reversed-phase high-performance liquid chromatographic (HPLC) method has been established for the determination of a newly developed anti-cancer agent desoxyepothilone B (dEpo B) in nude mice plasma. The sample preparation involved deproteination of 200 microl of plasma sample first, followed by liquid-liquid extraction of the resultant supernatant with chloroform. The compound taxol was used as the internal standard. Chromatographic separations were carried out on a 250 mm x 4.6 mm Zorbax SB-phenyl column with acetonitrile-0.25% orthophosphoric acid (50/50, v/v) as mobile phase and UV detection at 250 nm. For dEpo B and taxol at the concentration level of 10 microg/ml in nude mice plasma, the absolute extraction recoveries were 85.3 and 87.2%, respectively. The linear quantification range of the method was 0.1-100 microg/ml in nude mice plasma with linear correlation coefficients greater than 0.999. The within-day and between-day relative standard deviations (R.S.D.s) for dEpo B at 0.5, 2.5 and 10 microg/ml levels in nude mice plasma fell in the range of 2.8-4.8 and 1.5-4.6%, and the within-day and between-day recoveries were in the range of 96.5-101.7 and 97.7-101.2%, respectively.

• Authors: Zhi Wang, Hui Wang, Julie K Rhie, Joseph M Covey, Ping Liang, Shuyi Wang, Chun Wang, Yanxue Hu, Gautam Prasad, Li Nan, Donald L Hill, Ruiwen Zhang
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 42 Issue 2 Pg. 272-6 (Sep 18 2006) ISSN: 0731-7085 [Print] England
• PMID: 16616824 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Epothilones
• desoxyepothilone B

Topics

• Animals
• Antineoplastic Agents (blood, pharmacokinetics)
• Chromatography, High Pressure Liquid (methods)
• Epothilones (blood, pharmacokinetics)
• Mice
• Mice, Nude
• Reference Standards
• Reproducibility of Results
• Sensitivity and Specificity