Abstract | BACKGROUND AND OBJECTIVES: STUDY DESIGN/MATERIALS AND METHODS: A dorsal skinfold window chamber was installed surgically on female Sprague-Dawley rats. One milligram per kilogram of BPD solution was administered intravenously via a jugular venous catheter. Evaluated interventions were: control (no BPD, no light), PDT alone (576 nm, 16 minutes exposure time, 15 minutes post-BPD injection, 10 mm spot), PDL alone at 7 J/cm2 (585 nm, 1.5 ms pulse duration, 7 mm spot), PDL alone at 10 J/cm2, PDT/PDL (PDL at 7 J/cm2), and PDT/PDL (PDL at 10 J/cm2). To assess changes in microvascular blood flow, laser speckle imaging was performed before, immediately after, and 18 hours post-intervention. RESULTS: Epidermal irradiation was accomplished without blistering, scabbing or ulceration. A reduction in perfusion was achieved in all intervention groups. PDT/PDL at 7 J/cm2 resulted in the greatest reduction in vascular perfusion (56%). CONCLUSIONS: BPD PDT can achieve safe and selective vascular flow reduction. PDT/PDL can enhance diminution of microvascular blood flow. Our results suggest that PDT and PDT/PDL should be evaluated as alternative therapeutic options for treatment of hypervascular skin lesions including port wine stain birthmarks.
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Authors | Tia K Smith, Bernard Choi, Julio C Ramirez-San-Juan, J Stuart Nelson, Kathryn Osann, Kristen M Kelly |
Journal | Lasers in surgery and medicine
(Lasers Surg Med)
Vol. 38
Issue 5
Pg. 532-9
(Jun 2006)
ISSN: 0196-8092 [Print] United States |
PMID | 16615132
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2006 Wiley-Liss, Inc. |
Chemical References |
- Photosensitizing Agents
- Porphyrins
- Verteporfin
|
Topics |
- Animals
- Female
- Low-Level Light Therapy
- Microcirculation
- Models, Animal
- Photochemotherapy
- Photosensitizing Agents
(pharmacology)
- Porphyrins
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Regional Blood Flow
- Skin
(blood supply)
- Verteporfin
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