The transsulfuration pathway converts
homocysteine to
cysteine and represents the metabolic link between
antioxidant and methylation metabolism. The first and committing step in this pathway is catalyzed by
cystathionine beta-synthase (CBS), which is subject to complex regulation, including allosteric activation by the methyl donor,
S-adenosylmethionine (
AdoMet). In this study, we demonstrate that
methionine restriction leads to
a >10-fold decrease in CBS
protein levels, and pulse proteolysis studies reveal that binding of
AdoMet stabilizes the
protein against degradation by approximately 12 kcal/mol. These observations predict that under pathological conditions where
AdoMet levels are diminished, CBS, and therefore
glutathione levels, will be reduced. Indeed, we demonstrate this to be the case in a mouse model for spontaneous
steatohepatitis in which the gene for the MAT1A
isoenzyme encoding
AdoMet synthetase has been disrupted, and in human
hepatocellular carcinoma, where MAT1A is silenced. Furthermore, diminished CBS levels are associated with reduced cell viability in
hepatoma cells challenged with
tert-butyl hydroperoxide. This study uncovers a mechanism by which CBS is allosterically activated by
AdoMet under normal conditions but is destabilized under pathological conditions, for redirecting the metabolic flux toward
methionine conservation. A mechanistic basis for the coordinate changes in redox and methylation metabolism that are a hallmark of several complex diseases is explained by these observations.