[Inhibition effect of fludarabine on the growth of myelodysplastic syndrome cell line MUTZ-1 and its mechanism].

Abstract

OBJECTIVE:

To investigate the inhibition effect of fludarabine on the growth of human MDS-RAEB cell line MUTZ-1 and to explore the possible cellular and molecular mechanism.

METHODS:

The apoptosis of MUTZ-1 cells induced by fludarabine was studied by transmission electron microscope, MTT assay, DNA ladder test, flow cytometry and RT-PCR method.

RESULT:

Treatment with fludarabine remarkably inhibited the growth of MUTZ-1 cells, the 24 h IC(50), 48 h IC(50) and 72 h IC(50) of fludarabine for MUTZ-1 cells were 137.65 mg/L, 6.27 mg/L and 0.51 mg/L, respectively. Fludarabine inhibited the growth of MUTZ-1 cells in a dose-dependent and time-dependent manner. After treated by fludarabine (1 mg/L-16 mg/L)for 24 h, MUTZ-1 cells showed the typical features of apoptosis. After fludarabine treatment the mRNA expression of Bcl-2, Bax, survivin, XIAP, cIAP-1 and cIAP-2 was not changed, but the mitochondrial membrane potential (MMP) was decreased.

CONCLUSION:

With a certain range of dose fludarabine (1 mg/L-16 mg/L)could inhibit MUTZ-1 cell growth by inducing cells apoptosis. MMP may play a certain role in apoptosis of MUTZ-1 cells induced by fludarabine.

• Authors: Wen-jun Wu, Zhen Cai
• Journal: Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (Zhejiang Da Xue Xue Bao Yi Xue Ban) Vol. 35 Issue 2 Pg. 136-42 (03 2006) ISSN: 1008-9292 [Print] China
• PMID: 16610078 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• BIRC5 protein, human
• Inhibitor of Apoptosis Proteins
• Microtubule-Associated Proteins
• Neoplasm Proteins
• Proto-Oncogene Proteins c-bcl-2
• RNA, Messenger
• Survivin
• bcl-2-Associated X Protein
• Vidarabine
• fludarabine

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Cell Line, Tumor
• Humans
• Inhibitor of Apoptosis Proteins
• Microtubule-Associated Proteins (biosynthesis, genetics)
• Myelodysplastic Syndromes (pathology)
• Neoplasm Proteins (biosynthesis, genetics)
• Proto-Oncogene Proteins c-bcl-2 (biosynthesis, genetics)
• RNA, Messenger (biosynthesis, genetics)
• Survivin
• Vidarabine (analogs & derivatives, pharmacology)
• bcl-2-Associated X Protein (biosynthesis, genetics)