Chimeric peptide vaccine composed of B- and T-cell epitopes of human T-cell leukemia virus type 1 induces humoral and cellular immune responses and reduces the proviral load in immunized squirrel monkeys (Saimiri sciureus).

Abstract	A squirrel monkey model of human T-cell leukemia virus type 1 (HTLV-1) infection was used to evaluate the immunogenicity and protective efficacy of a chimeric peptide vaccine composed of a B-cell epitope from the envelope region (aa 175-218) and three HLA-A*0201-restricted cytotoxic T-lymphocyte epitopes derived from Tax protein (Tri-Tax). These selected Tax peptides induced secretion of gamma interferon (IFN-gamma) in peripheral blood mononuclear cells obtained from monkeys chronically infected with HTLV-1. After immunization, a high titre of antibodies and a high frequency of IFN-gamma-producing cells were detected against the Env and the Tri-Tax immunogens, but not against the individual Tax peptides. This might indicate that epitope(s) distinct from those recognized by humans are recognized by responder monkeys. After challenge, it was shown by competitive PCR that partial protection against HTLV-1 infection could be raised in immunized animals. Further studies should be developed to determine the duration of this protection.
Authors	Mirdad Kazanji, Jean-Michel Heraud, Fabrice Merien, Claudine Pique, Guy de Thé, Antoine Gessain, Steven Jacobson
Journal	The Journal of general virology (J Gen Virol) Vol. 87 Issue Pt 5 Pg. 1331-1337 (May 2006) ISSN: 0022-1317 [Print] England
PMID	16603536 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antibodies, Viral Epitopes, B-Lymphocyte Epitopes, T-Lymphocyte Gene Products, env Gene Products, tax HLA-A Antigens Vaccines, Synthetic Viral Vaccines Interferon-gamma
Topics	Animals Antibodies, Viral (blood) Drug Administration Schedule Enzyme-Linked Immunosorbent Assay Epitopes, B-Lymphocyte (immunology) Epitopes, T-Lymphocyte (immunology) Gene Products, env (immunology) Gene Products, tax (immunology) HLA-A Antigens HTLV-I Infections (blood, immunology, prevention & control) Human T-lymphotropic virus 1 (immunology) Immunization, Secondary Injections, Intramuscular Interferon-gamma (blood) Saimiri Vaccines, Synthetic (administration & dosage, immunology) Viral Vaccines (administration & dosage, immunology)

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