The goal of the study was to monitor the antioxidative effect of
stobadine derivative in the conditions of
ischemia-reperfusion of laboratory rat kidney tissue. The animals were divided by random selection into 5 groups (n = 10). The treated groups were given
stobadine derivate in peroral doses of 5, 10 and 20 mg/kg in 0.5 %
solution of
Avicel once a day; the placebo group was given only the
solution of
Avicel. The last group was an intact group (without
ischemia-reperfusion and without treatment). After conclusion of medication on the 15th day all animals were subjected to kidney tissue
ischemia (60 min.) followed by reperfusion (10 min.). All animals were subsequently exsanquined and single identification of superoxiddismutase, glutathion
peroxidase, total antioxidative capacity, and
malondialdehyde level in the blood were determined. Kidneys were recovered for histopathological examination. A statistically significant decrease of the superoxiddismutase and statistically significant increase of the
glutathione peroxidase catalytic activity in the treated groups compared to the groups of placebo and intact was discovered. There was also a statistically highly significant increase of total antioxidative capacity in the treated groups compared to the groups of placebo and intact. A statistically significant decrease of
malondialdehyde level was identified in the treated groups compared to the groups of placebo and intact. The results of biochemical examination show a protective antioxidative effect of
stobadine derivative. The results of histopathological examination support this assumption.