Abstract |
In somatostatinoma, a rare malignant somatostatin (SST)-secreting neoplasia, tumour regression is rarely observed, implying the need for novel antiproliferative strategies. Here, we characterized a long-term culture (SST-secreting cancer (SS-C cells)) established from a human somatostatinoma. High concentrations of SST and chromogranin A were released by SS-C cells and SST release was stimulated by depolarizing stimuli and inhibited by the SST analogue, octreotide. SS-C cells expressed mRNA for SST receptor (SSTR) subtypes 1, 2 and 4, being also able to bind native SST. Moreover, SS-C cells were positively stained with an antibody to SSTR2. SS-C cells also expressed interferon-gamma (IFN-gamma) receptor mRNA and measurable telomerase activity. Our findings indicate that in vitro exposure of SS-C cells to native SST-28, to octreotide, to IFN-gamma, or to 3'-azido-3'deoxythymidine (AZT), a telomerase inhibitor, results in inhibition of SS-C cell proliferation. Concomitant with growth inhibition, apoptosis was detected in SST-, octreotide-, IFN-gamma- or AZT-treated SS-C cell cultures. Taken together our results characterized native SST, SST analogues, IFN-gamma and a telomerase inhibitor as growth-inhibiting and proapoptotic stimuli in cultured human somatostatinoma cells. Based on these findings, the potential of SST analogues, IFN-gamma and AZT, alone or in combination, should be further explored in the medical treatment of somatostatinoma.
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Authors | G Galli, R Zonefrati, A Gozzini, C Mavilia, V Martineti, I Tognarini, G Nesi, T Marcucci, F Tonelli, M Tommasi, C Casini Raggi, P Pinzani, M L Brandi |
Journal | Endocrine-related cancer
(Endocr Relat Cancer)
Vol. 13
Issue 1
Pg. 79-93
(Mar 2006)
ISSN: 1351-0088 [Print] England |
PMID | 16601281
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-HIV Agents
- Antineoplastic Agents, Hormonal
- Chromogranin A
- Chromogranins
- RNA, Messenger
- Receptors, Somatostatin
- Zidovudine
- Somatostatin
- Interferon-gamma
- somatostatin receptor 2
- Telomerase
- Octreotide
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Topics |
- Adult
- Anti-HIV Agents
(pharmacology)
- Antineoplastic Agents, Hormonal
(pharmacology)
- Cell Proliferation
(drug effects)
- Chromogranin A
- Chromogranins
(metabolism)
- Female
- Humans
- Interferon-gamma
(genetics, pharmacology)
- Jejunal Neoplasms
(metabolism, pathology)
- Octreotide
(pharmacology)
- RNA, Messenger
- Receptors, Somatostatin
(metabolism)
- Somatostatin
(metabolism)
- Somatostatinoma
(metabolism, pathology)
- Telomerase
(antagonists & inhibitors, metabolism)
- Tumor Cells, Cultured
- Zidovudine
(pharmacology)
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