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Characterization of the functional and growth properties of long-term cell cultures established from a human somatostatinoma.

Abstract
In somatostatinoma, a rare malignant somatostatin (SST)-secreting neoplasia, tumour regression is rarely observed, implying the need for novel antiproliferative strategies. Here, we characterized a long-term culture (SST-secreting cancer (SS-C cells)) established from a human somatostatinoma. High concentrations of SST and chromogranin A were released by SS-C cells and SST release was stimulated by depolarizing stimuli and inhibited by the SST analogue, octreotide. SS-C cells expressed mRNA for SST receptor (SSTR) subtypes 1, 2 and 4, being also able to bind native SST. Moreover, SS-C cells were positively stained with an antibody to SSTR2. SS-C cells also expressed interferon-gamma (IFN-gamma) receptor mRNA and measurable telomerase activity. Our findings indicate that in vitro exposure of SS-C cells to native SST-28, to octreotide, to IFN-gamma, or to 3'-azido-3'deoxythymidine (AZT), a telomerase inhibitor, results in inhibition of SS-C cell proliferation. Concomitant with growth inhibition, apoptosis was detected in SST-, octreotide-, IFN-gamma- or AZT-treated SS-C cell cultures. Taken together our results characterized native SST, SST analogues, IFN-gamma and a telomerase inhibitor as growth-inhibiting and proapoptotic stimuli in cultured human somatostatinoma cells. Based on these findings, the potential of SST analogues, IFN-gamma and AZT, alone or in combination, should be further explored in the medical treatment of somatostatinoma.
AuthorsG Galli, R Zonefrati, A Gozzini, C Mavilia, V Martineti, I Tognarini, G Nesi, T Marcucci, F Tonelli, M Tommasi, C Casini Raggi, P Pinzani, M L Brandi
JournalEndocrine-related cancer (Endocr Relat Cancer) Vol. 13 Issue 1 Pg. 79-93 (Mar 2006) ISSN: 1351-0088 [Print] England
PMID16601281 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-HIV Agents
  • Antineoplastic Agents, Hormonal
  • Chromogranin A
  • Chromogranins
  • RNA, Messenger
  • Receptors, Somatostatin
  • Zidovudine
  • Somatostatin
  • Interferon-gamma
  • somatostatin receptor 2
  • Telomerase
  • Octreotide
Topics
  • Adult
  • Anti-HIV Agents (pharmacology)
  • Antineoplastic Agents, Hormonal (pharmacology)
  • Cell Proliferation (drug effects)
  • Chromogranin A
  • Chromogranins (metabolism)
  • Female
  • Humans
  • Interferon-gamma (genetics, pharmacology)
  • Jejunal Neoplasms (metabolism, pathology)
  • Octreotide (pharmacology)
  • RNA, Messenger
  • Receptors, Somatostatin (metabolism)
  • Somatostatin (metabolism)
  • Somatostatinoma (metabolism, pathology)
  • Telomerase (antagonists & inhibitors, metabolism)
  • Tumor Cells, Cultured
  • Zidovudine (pharmacology)

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