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Drug screening in urine by cloned enzyme donor immunoassay (CEDIA) and kinetic interaction of microparticles in solution (KIMS): a comparative study.

Abstract
Two commercially available drug-screening assays were evaluated: the Roche kinetic interaction of microparticles in solution (KIMS) assay and the Microgenics cloned enzyme donor immunoassay (CEDIA). Urine samples from known drug-abuse patients were analyzed for amphetamines, barbiturates, benzodiazepines, benzoylecgonine, cannabinoids, LSD, methadone and opiates. Samples with discordant findings for the two assays were analyzed by gas chromatography/mass spectrometry (GC/MS) or gas chromatography/electron capture detection (GC/ECD). Amphetamines showed 96.0% concordant results, with two false positive findings by CEDIA, three by KIMS and a further two false negatives by KIMS. Barbiturates showed 99.4% concordant results, with one false negative by KIMS. Benzodiazepines showed 97.4% concordant results, with two false negatives by KIMS (cutoff 100 microg/L, CEDIA cutoff 300 microg/L). Benzoylecgonine showed 17.8% concordant positive and 82.2% concordant negative results and no false finding by either assay. Cannabinoids showed 99.3% concordant results, with one sample negative by KIMS at a cutoff of 50 microg/L and positive by CEDIA (cutoff 25 microg/L). For LSD, 6.7% of findings were not in agreement. Methadone showed 97.5% concordant results, with two false positives by CEDIA, and one false positive and one false negative by KIMS. Opiates showed 96.9% concordant results, with no false KIMS results, but four false positives by CEDIA. The results indicate that the agreement of the CEDIA and KIMS results for the eight drugs is rather good (93.3-100%).
AuthorsLutz Schwettmann, Wolf-Rüdiger Külpmann, Christian Vidal
JournalClinical chemistry and laboratory medicine (Clin Chem Lab Med) Vol. 44 Issue 4 Pg. 479-87 ( 2006) ISSN: 1434-6621 [Print] Germany
PMID16599844 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Amphetamines
  • Barbiturates
  • Cannabinoids
  • Illicit Drugs
  • Narcotics
  • Reagent Kits, Diagnostic
  • Benzodiazepines
  • benzoylecgonine
  • Lysergic Acid Diethylamide
  • Cocaine
  • Methadone
Topics
  • Amphetamines (urine)
  • Barbiturates (urine)
  • Benzodiazepines (urine)
  • Cannabinoids (urine)
  • Chromatography, Gas
  • Cocaine (analogs & derivatives, urine)
  • Diagnostic Errors
  • Humans
  • Illicit Drugs (urine)
  • Immunoassay (methods, standards)
  • Lysergic Acid Diethylamide (urine)
  • Methadone (urine)
  • Narcotics (urine)
  • Reagent Kits, Diagnostic
  • Substance Abuse Detection (methods)

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