Hypobaric hypoxia affects endogenous levels of alpha-keto acids in murine heart ventricles.

Abstract

Alpha-keto acids have recently been identified as potent regulators of cellular adaptations to hypoxia. Their actual intracellular concentrations under such conditions are unknown. Here, we determined concentrations of alpha-ketobutyrate, alpha-ketoglutarate, alpha-ketoisocaproate, alpha-ketoisovalerate, alpha-keto-beta-methylvalerate, phenylpyruvate, and pyruvate by a recently developed ultra-sensitive fluorescence HPLC method in ventricular myocardium of mice exposed to hypobaric hypoxia for up to 3 weeks. We observed characteristic alterations of cardiac alpha-keto acid concentrations that are specific for individual alpha-keto acids, show significant side differences (right versus left ventricles), and are suited to trigger some of the cardiac metabolic and structural adaptations to chronic hypoxia.

• Authors: Jörg Mühling, Renate Paddenberg, Gunter Hempelmann, Wolfgang Kummer
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 342 Issue 3 Pg. 935-9 (Apr 14 2006) ISSN: 0006-291X [Print] United States
• PMID: 16598846 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Keto Acids
• Phenylenediamines
• Mercaptoethanol
• 1,2-diaminobenzene

Topics

• Animals
• Body Weight
• Cell Hypoxia (physiology)
• Female
• Heart Ventricles (metabolism)
• Keto Acids (metabolism)
• Male
• Mercaptoethanol
• Mice
• Phenylenediamines