| Abstract | MDA-MB-231 human breast cancer cells belong to a highly invasive metastatic cell line that depends on phospholipase D (PLD) activity for survival when deprived of serum growth factors. In response to the stress of serum withdrawal, there is a rapid and dramatic increase in PLD activity. Concomitant with increased PLD activity, there was an increase in the ability of MDA-MB-231 cells to both migrate and invade Matrigel. The ability of MDA-MB-231 cells to both migrate and invade Matrigel was dependent on both PLD and mTOR, a downstream target of PLD signals. Serum withdrawal also led to a PLD-dependent increase in the expression of the stress factor, hypoxia-inducible factor-1alpha. These data reveal that PLD survival signals not only prevent apoptosis but also stimulate cell migration and invasion, linking the ability to suppress apoptosis with the ability to metastasize. |
| Authors | Yang Zheng, Vanessa Rodrik, Alfredo Toschi, Ming Shi, Li Hui, Yingjie Shen, David A Foster
(Affiliation: Department of Biological Sciences, Hunter College of the City University of New York, New York, New York 10021, USA.)
|
| Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 281
Issue 23
Pg. 15862-8
(Jun 9 2006)
ISSN: 0021-9258 United States |
| PMID | 16595654
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
| Chemical References |
- Culture Media, Serum-Free
- Phospholipase D
|
| Topics |
- Cell Line, Tumor
- Cell Movement
- Cell Survival
- Culture Media, Serum-Free
- Humans
- Neoplasm Metastasis
- Oxidative Stress
- Phospholipase D
(metabolism)
|
