Prevalence and clinico-serological correlations of anti-alpha-enolase, anti-C1q, and anti-dsDNA antibodies in patients with systemic lupus erythematosus.

Abstract

OBJECTIVE:

To evaluate the prevalence and clinico-serological correlations of anti-enolase, anti-C1q, and anti-dsDNA antibodies in patients with systemic lupus erythematosus (SLE).

METHODS:

Sixty-eight sera randomly obtained from SLE patients were examined. Anti-alpha-enolase antibodies were detected by immunoblot on recombinant protein; anti-C1q and anti-dsDNA antibodies were detected using an ELISA.

RESULTS:

Anti-alpha-enolase, anti-C1q, and anti-dsDNA antibodies were positive in 21%, 62%, and 63% of patients, respectively. A correlation was found between anti-dsDNA and anti-C1q antibodies, while anti-enolase antibodies did not correlate with the other 2 specificities. Anti-alpha-enolase antibodies were not correlated with any of the clinical and serological variables examined. Anti-C1q antibodies were correlated with ECLAM score, leukopenia, complement levels, and active renal involvement. Anti-dsDNA antibodies correlated with arthritis, leukopenia, complement levels, and the presence of renal involvement, independent of activity. In patients with active renal disease anti-dsDNA antibodies were correlated with a poor renal outcome, occurring after a mean period of 24 months.

CONCLUSION:

These data suggest the association of anti-C1q antibodies with disease flares and active renal disease in SLE. The observed association of anti-dsDNA antibodies and renal disease was expected. Further analysis is required to fully assess the clinical significance of anti-a-enolase antibodies.

• Authors: Marta Mosca, Daniele Chimenti, Federico Pratesi, Chiara Baldini, Consuelo Anzilotti, Stefano Bombardieri, Paola Migliorini
• Journal: The Journal of rheumatology (J Rheumatol) Vol. 33 Issue 4 Pg. 695-7 (Apr 2006) ISSN: 0315-162X [Print] Canada
• PMID: 16583471 (Publication Type: Journal Article)

Chemical References

• Antibodies, Antinuclear
• Autoantibodies
• Biomarkers, Tumor
• DNA-Binding Proteins
• Tumor Suppressor Proteins
• Complement C1q
• DNA
• ENO1 protein, human
• Phosphopyruvate Hydratase

Topics

• Antibodies, Antinuclear (blood, immunology)
• Autoantibodies (blood, immunology)
• Biomarkers, Tumor (blood, immunology)
• Complement C1q (analysis, immunology)
• DNA (immunology)
• DNA-Binding Proteins (blood, immunology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Immunoblotting
• Lupus Erythematosus, Systemic (blood, diagnosis, immunology)
• Male
• Phosphopyruvate Hydratase (blood, immunology)
• Prognosis
• Severity of Illness Index
• Tumor Suppressor Proteins (blood, immunology)