Evaluation of methyl inosine monophosphate (MIMP) and peramivir activities in a murine model of lethal influenza A virus infection.

Abstract

An inbred murine model (BALB/c) was utilized to assess the protective effect of the immunomodulator methyl inosine 5'-monophosphate (MIMP) against infection with influenza A/PR/8/34 (H1N1) virus. Contrary to the data reported for outbred mice (NMRI) infected with the aerosolized virus (Masihi, Hadden, 2003. J. Int. Immunopharmacol. 3, 1205-1215), there were no improvements in the outcomes of infection in the inbred animals treated with MIMP intranasally 1 day before the challenge and/or orally after the challenge for 5 days (up to 10 mg/kg/day). Nevertheless, complete protection against lethality was afforded by the treatment with the neuraminidase inhibitor peramivir given once daily for 5 days after the challenge (10 mg/kg/day). We speculate that the rapid progression of the disease in inbred mice caused by the intranasal challenge may render the MIMP-treatment ineffective. Our results emphasize the need for careful consideration of murine strains and routes of virus challenge in the design of experiments utilizing lethal influenza virus infection.

• Authors: Vasiliy P Mishin, Frederick G Hayden, Kathy L Signorelli, Larisa V Gubareva
• Journal: Antiviral research (Antiviral Res) Vol. 71 Issue 1 Pg. 64-8 (Aug 2006) ISSN: 0166-3542 [Print] Netherlands
• PMID: 16581141 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Acids, Carbocyclic
• Antiviral Agents
• Cyclopentanes
• Guanidines
• Inosine Monophosphate
• methyl inosine monophosphate
• peramivir

Topics

• Acids, Carbocyclic
• Age Factors
• Animals
• Antiviral Agents (pharmacology)
• Body Weight
• Cyclopentanes (pharmacology)
• Disease Models, Animal
• Female
• Guanidines (pharmacology)
• Influenza A Virus, H1N1 Subtype (growth & development)
• Inosine Monophosphate (analogs & derivatives, pharmacology)
• Mice
• Mice, Inbred BALB C
• Orthomyxoviridae Infections (drug therapy, virology)