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Connexin expression in mouse lung tumor.

Abstract
Gap junction intercellular communication (GJIC) is considered to play roles in regulation of homeostasis, development and differentiation of many tissues. In the present study, using reverse transcription-polymerase chain reaction (RT-PCR) and in situ RT-PCR, we examined expression of Connexins (Cx26, 32, 37, 40, 43 and 45) in the normal lung and lung tumors of mice to determine whether their expressions change during lung tumorigenesis. Cx26, 32 and 40 were expressed similarly in the normal lung tissue and tumors with smaller size (0.5-1.5mm) though expression of Cx32 and 40 decreased in tumors with larger size (>2.5mm). Cx26 was undetectable in larger size tumors. Cx37 and 45 were expressed in both normal lung and larger size tumors but no expression was seen in smaller size tumors. Cx43 was similarly detectable in normal lung, smaller size tumor and larger size tumor, but western blotting showed that Cx43 was phosphorylated during lung tumorigenesis. Thus, it is likely that alteration of expression of these Cx may be involved in expression of the neoplastic phenotype.
AuthorsNaoko Udaka, Yohei Miyagi, Takaaki Ito
JournalCancer letters (Cancer Lett) Vol. 246 Issue 1-2 Pg. 224-9 (Feb 08 2007) ISSN: 0304-3835 [Print] Ireland
PMID16580773 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 4-nitroquinolone-1-oxide
  • Connexin 43
  • Connexins
  • Quinolones
  • RNA, Messenger
  • connexin 32
  • connexin 37
  • connexin 40
  • connexin 45
  • Connexin 26
  • 4-Nitroquinoline-1-oxide
Topics
  • 4-Nitroquinoline-1-oxide
  • Adenocarcinoma (chemically induced, genetics, metabolism)
  • Animals
  • Blotting, Western
  • Connexin 26
  • Connexin 43 (genetics, metabolism)
  • Connexins (genetics, metabolism)
  • Gene Expression Regulation, Neoplastic
  • Lung (chemistry, metabolism)
  • Lung Neoplasms (chemically induced, genetics, metabolism)
  • Male
  • Mice
  • Mice, Inbred A
  • Phosphorylation
  • Quinolones
  • RNA, Messenger (genetics, metabolism)
  • Reverse Transcriptase Polymerase Chain Reaction (methods)

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