Syndecan-2 is expressed in the microvasculature of gliomas and regulates angiogenic processes in microvascular endothelial cells.

Abstract

Angiogenesis is the formation of new blood vessels from the existing vasculature and is necessary for tumor growth. Syndecan-2 (S2) is highly expressed in the microvasculature of mouse gliomas. When S2 expression was down-regulated in mouse brain microvascular endothelial cells (MvEC), this inhibited cell motility and reduced the formation of capillary tube-like structures in vitro. Pro-angiogenic growth factors and enzymes up-regulated during glioma tumorigenesis stimulated shedding of the S2 ectodomain from endothelial cells in vitro. The effect of shed S2 on angiogenic processes was investigated by incorporating recombinant S2 ectodomain (S2ED) into in vitro angiogenesis assays. S2ED promoted membrane protrusion, migration, capillary tube formation, and cell-cell interactions. We therefore propose that S2 is necessary for angiogenesis of MvEC, proangiogenic factors expressed during glioma progression regulate S2 shedding, and shed S2 ectodomain may increase endothelial cell angiogenic processes.

• Authors: Constance Y Fears, Candece L Gladson, Anne Woods
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 281 Issue 21 Pg. 14533-6 (May 26 2006) ISSN: 0021-9258 [Print] United States
• PMID: 16574663 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Membrane Glycoproteins
• Proteoglycans
• Recombinant Proteins
• SDC2 protein, human
• Sdc2 protein, mouse
• Syndecan-2

Topics

• Animals
• Brain Neoplasms (metabolism)
• Cattle
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Endothelium, Vascular (metabolism)
• Gene Expression Regulation
• Glioma (metabolism)
• Humans
• Membrane Glycoproteins (biosynthesis)
• Mice
• Microcirculation
• Neovascularization, Pathologic
• Proteoglycans (biosynthesis)
• Recombinant Proteins (chemistry)
• Syndecan-2