Identification and characterization of HIV-1 epitopes presented by HLA-A*2603: comparison between HIV-1 epitopes presented by A*2601 and A*2603.

Abstract

Human leukocyte antigen (HLA)-A*26 is one of the alleles associated with a slow progression to AIDS. Identification and characterization of HIV-1-specific epitopes presented by this allele are necessary for studies on the immunopathogenesis of AIDS and vaccine development in Asia, where three HLA-A*26 subtypes are frequently found. In the present study, we sought to identify HLA-A*2603-restricted HIV-1 epitopes by using reverse immunogenetics and to compare them with HLA-A*2601-restricted ones recently identified. We found that 31 of 110 HIV-1 peptides bound to HLA-A*2603 and that only two peptides (Gag169-177 and Env63-72) induced specific CD8+T cells by stimulating peripheral blood mononuclear leukocytes from HIV-1-infected individuals carrying HLA-A*2603. The specific cytotoxic T lymphocyte clones killed HIV-1 recombinant vaccinia-infected cells, indicating that these two peptides were naturally occurring peptides presented by HLA-A*2603. Gag169-177-specific CD8+T cells were frequently detected in both HLA-A*2601+ and -A*2603+ individuals with chronic HIV-1 infection, whereas Env63-72-specific ones were frequently detected only in the HLA-A*2603+ individuals. Gag169-177 peptide bound equally to both HLA-A*26 antigens, whereas Env63-72 peptide bound to A*2603 much more strongly than to A*2601. These findings suggest that the relative affinity of these peptides for the HLA-A*26 subtypes determines whether these peptides are recognized as epitopes in HIV-1-infected individuals carrying these alleles.

• Authors: Yuka Kawashima, Manami Satoh, Shinichi Oka, Masafumi Takiguchi
• Journal: Human immunology (Hum Immunol) Vol. 66 Issue 11 Pg. 1155-66 (Nov 2005) ISSN: 0198-8859 [Print] United States
• PMID: 16571416 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Epitopes
• HLA-A Antigens
• HLA-A*26 antigen
• HLA-A*26:01 antigen
• HLA-A*26:03 antigen
• HLA-A2 Antigen
• Peptides
• Viral Proteins

Topics

• Animals
• Antigen Presentation (immunology)
• CD8-Positive T-Lymphocytes (immunology, metabolism, virology)
• Cell Line, Tumor
• Cytotoxicity Tests, Immunologic
• Epitope Mapping
• Epitopes (chemistry, immunology, metabolism)
• HIV-1 (immunology)
• HLA-A Antigens (immunology, metabolism)
• HLA-A2 Antigen (immunology, metabolism)
• Humans
• Mice
• Peptides (immunology, metabolism)
• Viral Proteins (immunology, metabolism)