[Effects of aminoguanidine on the lung injury induced by the total hepatic ischemia-reperfusion in rats].

Abstract

OBJECTIVE:

To investigate the effects of aminoguanidine on the lung injury induced by the total hepatic ischemia-reperfusion in rats.

METHODS:

The total hepatic ischemia-reperfusion model was built after blocking of the hepatic porta, suprahepatic and infrahepatic vena cava. Ninety Sprague-Dawley rats were assigned randomly into 3 groups: Sham operation group (Group A, n=30); total hepatic ischemia group (Group B, n=30); and aminoguanidine treatment group (Group C, n=30). Each group was subdivided randomly into 3 subgroups (n=10) according to different time phases: 20 minutes after the total hepatic vascular exclusion (T0), 4 hours after the reperfusion (T1), and 48 hours after the survival Group A and Group B were intravenously injected with normal saline ( mL/kg) while Group C was injected with aminoguanidine (20 mg/kg) dissolved in normal saline (1 mL/kg) 10 minutes before the open of the abdomin. The levels of portal blood nitric oxide ( O) endotoxin ( ET), tumor necrosis factor-alpha (TNF-alpha at T0 and T1 were detected; 48 hours survival rates and the lung wet/dry weight ratio were counted; and the histological changes of the lung tissues were observed.

RESULTS:

Compared with Group A, the levels of portal vein NO, ET, and TNF-alpha T0 and T1 in Group B and Group C were significantly higher (P < 0.05 or P < 0.01). But those indexes of Group C were lower than those of Group B (P < 0.05). The 48-hour survival rate in Group C was higher than that in Group B (P < 0.05). The lung wet/dry weight ratio in Group C was lower than in Group B (P < 0.05) and the histological change of Group C was slighter than that in Group B.

CONCLUSION:

Aminoguanidine has the protective effects on the lungs against the total hepatic ischemia-reperfusion induced injury.

• Authors: De-quan Cao, Yan-ping Chen, Ye-tian Chang, Yong-guo Li
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 31 Issue 1 Pg. 94-6 (Feb 2006) ISSN: 1672-7347 [Print] China
• PMID: 16562685 (Publication Type: Journal Article)

Chemical References

• Enzyme Inhibitors
• Guanidines
• Nitric Oxide Synthase Type II
• pimagedine

Topics

• Animals
• Enzyme Inhibitors (pharmacology)
• Female
• Guanidines (pharmacology)
• Liver (blood supply)
• Male
• Nitric Oxide Synthase Type II (antagonists & inhibitors)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (prevention & control)
• Respiratory Distress Syndrome (prevention & control)