Abstract |
DNA of invasive ductal carcinomas from five women was analyzed for structural alterations in the purine nucleotides using gas chromatography-mass spectrometry with selected ion monitoring. The results were compared to those for a normal DNA control. The carcinoma DNA showed dramatically higher concentrations of the base modifications 8-hydroxy-guanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine, and 8-hydroxyadenine. For example, the concentration of total identified base modifications represented a more than 9-fold increase over the control value. Base modifications of this type, which arise from radical-induced hydroxylation and cleavage reactions of the purine ring, likely play a major role in initiation and probably contribute to the further transformation of neoplastic cells in cancer of the female breast.
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Authors | D C Malins, R Haimanot |
Journal | Cancer research
(Cancer Res)
Vol. 51
Issue 19
Pg. 5430-2
(Oct 01 1991)
ISSN: 0008-5472 [Print] United States |
PMID | 1655250
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Pyrimidines
- 2,6-diamino-4-hydroxy-5-formamidopyrimidine
- 8-hydroxyadenine
- 8-hydroxyguanine
- Guanine
- DNA
- Adenine
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Topics |
- Adenine
(analogs & derivatives, analysis)
- Breast Neoplasms
(genetics)
- Carcinoma, Intraductal, Noninfiltrating
(genetics)
- Cell Transformation, Neoplastic
(genetics)
- Chromatography, Gas
- DNA
(analysis)
- Female
- Guanine
(analogs & derivatives, analysis)
- Humans
- Hydroxylation
- Mass Spectrometry
- Pyrimidines
(analysis)
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