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Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation.

Abstract
The plasma membrane H+-ATPase plays an important role in the plant response to nutrient and environmental stresses. However, the involvement of plant root plasma membrane H+-ATPase in adaptation to phosphate (P) starvation is not yet fully elucidated. In this study, experiments were performed with soybean roots in low-P nutrient solution (10 microM). Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%, while vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed it. These results suggested that P uptake might be regulated via the modulation of the activity of plasma membrane H+-ATPase under P starvation. The relationship between P uptake and the activity of plasma membrane H+-ATPase was examined further by using plasma membrane H+-ATPase transgenic Arabidopsis thaliana under low-P conditions. Transgenic plants absorbed more P compared with wild-type Arabidopsis. Results from real-time RT-PCR, western-blotting and immunolocalization analysis indicated that the increase in activity of the plasma membrane H+-ATPase by P starvation was caused by its transcriptional and translational regulation. A higher expression was observed at the translational level than at the transcriptional level. P starvation could induce a transient increase of endogenous indole-3-acetic acid (IAA) in soybean roots. The exogenous application of IAA stimulated the activity of plasma membrane H+-ATPase and P uptake, while naphthylphthalamic acid (NPA), an IAA transport inhibitor, blocked IAA effects. Taken together, these results suggested an involvement of root plasma membrane H+-ATPase in the adaptation of soybean to P starvation. IAA might be involved in signal transduction of P starvation by activating the plasma membrane H+-ATPase in soybean roots.
AuthorsHong Shen, Jianhong Chen, Zhanyi Wang, Cunyi Yang, Takara Sasaki, Yoko Yamamoto, Hideaki Matsumoto, Xiaolong Yan
JournalJournal of experimental botany (J Exp Bot) Vol. 57 Issue 6 Pg. 1353-62 ( 2006) ISSN: 0022-0957 [Print] England
PMID16547127 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Indoleacetic Acids
  • Phosphorus
  • Proton-Translocating ATPases
Topics
  • Adaptation, Physiological
  • Cell Membrane (metabolism)
  • Fluorescent Antibody Technique
  • Indoleacetic Acids (metabolism)
  • Phosphorus (metabolism)
  • Plant Roots (enzymology, physiology)
  • Plants, Genetically Modified
  • Proton-Translocating ATPases (metabolism)
  • Signal Transduction
  • Soybeans (enzymology, genetics, physiology)

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