Abstract | BACKGROUND & OBJECTIVE: METHODS: The expression of GHR was confirmed in Bel-7402 cells by radioligand assay. After transplantation of Bel-7402 hepatic carcinoma in liver, 32 nude mice were randomly divided into 4 groups. Except 1 control group, 3 experimental groups were injected with different dosages of rhGH [0.5, 1.0, and 2.0 U . (kg.day)(-1), respectively] subcutaneously 2 weeks after tumor transplantation, once a day for 2 weeks. All nude mice were killed at the end of the experiment to observe the growth of xenografts. The expression of proliferating cell nuclear antigen ( PCNA) in xenografts was examined by immunochemistry. RESULTS: The tumor weight was significantly heavier and the tumor volume was significantly larger in 1.0 U . (kg.day)(-1) rhGH and 2.0 U . (kg.day)(-1) rhGH groups than in control group (P<0.05), but no significant difference was found between 0.5 U . (kg.day)(-1) rhGH group and control group (P>0.05). Labeling index (LI) of PCNA was significantly higher in each experimental group than in control group (P<0.05), and significant differences were also found among the 3 experimental groups (P<0.05). The dosage of rhGH was positively correlated to LI of PCNA in tumor tissues (r=0.779, P<0.001). CONCLUSIONS: rhGH could enhance the growth of Bel-7402 hepatic carcinoma xenografts in nude mice. Its effect positively correlates with rhGH dosage.
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Authors | Jian-Ping Liu, Tao Chen, Yun-Biao Ling, Xiao-Xuan Chen, Qing-Jia Ou |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 25
Issue 3
Pg. 292-6
(Mar 2006)
China |
PMID | 16536981
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Proliferating Cell Nuclear Antigen
- Human Growth Hormone
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Topics |
- Animals
- Carcinoma, Hepatocellular
(metabolism, pathology)
- Cell Line, Tumor
- Human Growth Hormone
(pharmacology)
- Humans
- Liver Neoplasms
(metabolism, pathology)
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neoplasm Transplantation
- Proliferating Cell Nuclear Antigen
(metabolism)
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