N-myristoyltransferase 2 expression in human colon cancer: cross-talk between the calpain and caspase system.

Abstract

A number of viral and eukaryotic proteins which undergo a lipophilic modification by the enzyme N-myristoyltransferase (NMT: NMT1 and NMT2) are required for signal transduction and regulatory functions. To investigate whether NMT2 contributes to the pathogenesis of colorectal carcinoma, we observed a higher expression of NMT2 in most of the cases of cancerous tissues compared to normal tissues (84.6% of cases; P < 0.05) by Western blot analysis. Furthermore, protein-protein interaction of NMTs revealed that m-calpain interacts with NMT1 while caspase-3 interacts with NMT2. Our findings provide the first evidence of higher expression of NMT2 in human colorectal adenocarcinomas and the interaction of both forms of NMT with various signaling molecules.

• Authors: Ponniah Selvakumar, Erin Smith-Windsor, Keith Bonham, Rajendra K Sharma
• Journal: FEBS letters (FEBS Lett) Vol. 580 Issue 8 Pg. 2021-6 (Apr 03 2006) ISSN: 0014-5793 [Print] England
• PMID: 16530191 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proto-Oncogene Proteins c-bcl-2
• Tumor Suppressor Protein p53
• Acyltransferases
• glycylpeptide N-tetradecanoyltransferase
• Peptide Hydrolases
• Calpain
• Caspases

Topics

• Acyltransferases (metabolism)
• Calpain (metabolism)
• Caspases (metabolism)
• Colonic Neoplasms (enzymology, metabolism)
• Gene Expression Regulation, Neoplastic
• Humans
• Peptide Hydrolases (metabolism)
• Protein Binding
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Tumor Cells, Cultured
• Tumor Suppressor Protein p53 (metabolism)