Abstract |
Human Bub1 plays an important role at the spindle assembly check-point to prevent cell cycle progression following spindle damage. We examined the expression of Bub1 mRNA and protein in 21 human salivary gland tumors (7 pleomorphic adenomas, 2 warthin tumors, 5 mucoepidermoid carcinomas, 3 adenoid cystic carcinomas and 4 acinic cell carcinomas) and 3 normal submandibular glands using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) or western blotting. The mean expression levels of Bub1 mRNA and protein were higher in malignant tumors (0.12+/-0.028/1.75+/-0.53) than normal submandibular glands (0.042+/-0.014/0.19+/-0.044) and benign tumors (0.058+/-0.01/0.97+/-0.44). We found a significant association between the level of Bub1 mRNA/ protein expression and clinical stage in malignant tumors (Mann-Whitney U test, p=0.019/p=0.016). We analyzed its relation with the proliferative activity monitored by the Ki-67 labeling index by immunohistochemistry as well as the expression of proliferating cell nuclear antigen ( PCNA) by Western blotting. A significant correlation was found between Bub1 mRNA/ protein expression and the Ki-67 labeling index in salivary gland tumors (Spearman's correlation coefficient by rank test, p=0.026/p=0.002). These results indicate that increased expression of the human Bub1 gene is closely linked to abnormal cell proliferation in malignant conditions.
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Authors | Hideo Shigeishi, Shingo Yoneda, Masayuki Taki, Takeshi Nobumori, Kouji Ohta, Koichiro Higashikawa, Wataru Yasui, Nobuyuki Kamata |
Journal | Oncology reports
(Oncol Rep)
Vol. 15
Issue 4
Pg. 933-8
(Apr 2006)
ISSN: 1021-335X [Print] Greece |
PMID | 16525682
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Ki-67 Antigen
- Proliferating Cell Nuclear Antigen
- RNA, Messenger
- Protein Kinases
- BUB1 protein, human
- Bub1 spindle checkpoint protein
- Protein Serine-Threonine Kinases
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Topics |
- Adenolymphoma
(genetics, metabolism, pathology)
- Adenoma, Pleomorphic
(genetics, metabolism, pathology)
- Blotting, Western
- Carcinoma, Acinar Cell
(genetics, metabolism, pathology)
- Carcinoma, Adenoid Cystic
(genetics, metabolism, pathology)
- Carcinoma, Mucoepidermoid
(genetics, metabolism, pathology)
- Cell Proliferation
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Ki-67 Antigen
(analysis)
- Neoplasm Staging
- Proliferating Cell Nuclear Antigen
(analysis)
- Protein Kinases
(analysis, genetics)
- Protein Serine-Threonine Kinases
- RNA, Messenger
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Salivary Gland Neoplasms
(genetics, metabolism, pathology)
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