| Abstract | Elevated expression of protein casein kinase II (CKII) stimulated basal phospholipase D (PLD) activity as well as PMA-induced PLD activation in human U87 astroglioma cells. Moreover, CKII-selective inhibitor, emodin and apigenin suppressed PMA-induced PLD activation in a dose-dependent manner as well as basal PLD activity, suggesting the involvement of CKII in the activation of both PLD1 and PLD2. CKII was associated with PLD1 and PLD2 in co-transfection experiments. Furthermore, CKII induced serine/threonine phosphorylation of PLD2 in vivo, and the multiple regions of PLD2 were phosphorylated by CKII in vitro kinase assay using glutathione S-transferase-PLD2 fusion protein fragments. Elevated expression of CKII or PLD increased cell proliferation but pretreatment of cells with 1-butanol suppressed CKII-induced cell proliferation. These results suggest that CKII is involved in proliferation of U87 cells at least in part, through stimulation of PLD activity. |
| Authors | Bong-Hyun Ahn, Gyesik Min, Yoe-Sik Bae, Young-Seuk Bae, Do Sik Min
(Affiliation: Cardiovascular Branch National Heart, Lung and Blood Institute (NHLBI), NIH Bldg 10/CRC 5-3288, 10 Center Drive, Bethesda, MD 20892, USA.)
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| Journal | Experimental & molecular medicine
(Exp Mol Med)
Vol. 38
Issue 1
Pg. 55-62
(Feb 28 2006)
ISSN: 1226-3613 Korea (South) |
| PMID | 16520553
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Enzyme Inhibitors
- Recombinant Fusion Proteins
- Tetradecanoylphorbol Acetate
- 1-Butanol
- Glutathione Transferase
- Casein Kinase II
- Phospholipase D
|
| Topics |
- 1-Butanol
(pharmacology)
- Astrocytoma
(enzymology, metabolism, pathology)
- Blotting, Western
- Casein Kinase II
(analysis, pharmacology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Enzyme Activation
- Enzyme Inhibitors
(pharmacology)
- Glutathione Transferase
(metabolism)
- Humans
- Kinetics
- Phospholipase D
(genetics, metabolism)
- Phosphorylation
(drug effects)
- Precipitin Tests
- Recombinant Fusion Proteins
(metabolism)
- Tetradecanoylphorbol Acetate
(pharmacology)
|
