Abstract | BACKGROUND: METHODS AND RESULTS: CXCR1, a type of chemokine receptor, was over-expressed in endometriotic tissue. The high translation of the receptor and its ligand, interleukin (IL-8), in endometriotic tissue was then demonstrated by immunochemistry. Estradiol and 2,3,7,8-tetrachlorodibenzo-p-dioxin ( TCDD) alone inhibited expression of CXCR1, whereas the combination of estradiol with TCDD up-regulated the expression. TCDD promoted IL-8 secretion by human pelvic mesothelial cells (HPMC), and 17beta-estradiol magnified the stimulatory effect. Both 17beta-estradiol and TCDD alone inhibited IL-8 secretion of U937 (a cell line of monocyte), but combination of 17beta-estradiol and TCDD had no further inhibitory effect. The co-culture of endometrial stromal cells (ESC) with HPMC produced more IL-8 than respective or total production of either of the cells alone, and estradiol played a synergistic stimulatory role with TCDD in IL-8 secretion of the co-culture. Interaction of HPMC and the monocytes significantly stimulated IL-8 secretion, suggesting a main resource of IL-8 in peritoneal cavity with endometriosis. TCDD promoted IL-8 secretion by HPMC-U937 co-culture, but exerted a contrary effect for IL-8 secretion when combined with estradiol. CONCLUSION:
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Authors | Ying-Li Shi, Xue-Zhen Luo, Xiao-Yong Zhu, Ke-Qin Hua, Ying Zhu, Da-Jin Li |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 21
Issue 4
Pg. 870-9
(Apr 2006)
ISSN: 0268-1161 [Print] England |
PMID | 16517565
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Environmental Pollutants
- Interleukin-8
- Polychlorinated Dibenzodioxins
- Receptors, Interleukin-8A
- Estradiol
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Topics |
- Adult
- Cells, Cultured
- Coculture Techniques
- Drug Synergism
- Endometriosis
(immunology, pathology)
- Endometrium
(drug effects, immunology, pathology)
- Environmental Pollutants
(pharmacology)
- Estradiol
(pharmacology)
- Female
- Gene Expression Regulation
(drug effects)
- Humans
- Immunohistochemistry
- Interleukin-8
(genetics, metabolism)
- Middle Aged
- Polychlorinated Dibenzodioxins
(pharmacology)
- Receptors, Interleukin-8A
(genetics, metabolism)
- Signal Transduction
- U937 Cells
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