Characterization of the Sendai virus V protein with an anti-peptide antiserum.

Abstract

The Sendai virus V protein, which is a fusion of the P and V ORFs of the P gene, was characterized with antisera to a portion of the V ORF and compared to the P protein. The only property found in common with P is that V is also highly phosphorylated, and this is so even when these proteins are expressed independently of the other viral proteins. Otherwise, V was not found in virions, was not strongly associated with viral nucleocapsids like P, and anti-V had no effect on viral RNA synthesis in vitro under conditions where anti-P was highly inhibitory. The available evidence suggests that V may play a role in RNA synthesis, but it is not an essential one like that of the P protein.

• Authors: J Curran, M de Melo, S Moyer, D Kolakofsky
• Journal: Virology (Virology) Vol. 184 Issue 1 Pg. 108-16 (Sep 1991) ISSN: 0042-6822 [Print] United States
• PMID: 1651586 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Antigen-Antibody Complex
• Peptides
• RNA, Messenger
• RNA, Viral
• Ribonucleoproteins
• Viral Proteins

Topics

• Amino Acid Sequence
• Animals
• Antibodies
• Antigen-Antibody Complex
• Cell Line
• Electrophoresis, Polyacrylamide Gel
• Fluorescent Antibody Technique
• Genes, Viral
• Molecular Sequence Data
• Molecular Weight
• Open Reading Frames
• Parainfluenza Virus 1, Human (genetics)
• Peptides (chemical synthesis, immunology)
• Phosphorylation
• RNA, Messenger (genetics)
• RNA, Viral (genetics)
• Ribonucleoproteins (genetics, isolation & purification)
• Viral Proteins (analysis, genetics, immunology)