Abstract |
An HPLC-DAD-ESI-MSn method was developed for simultaneous analysis of the chemical constituents in ' SHUANGDAN' granules, a newly developed drug widely used for treating cardiovascular disease. The chromatographic separation were performed on a Zorbax Stable Bond C18 column (4.6 mmx250 mm, 5 microm) with water with 0.5% acetic acid (A) and acetonitrile (B) as mobile phase. According to the characteristic UV absorption profile, the information of molecular weight and structure provided by ESI-MSn, 29 constituents which attributed to Radix Salviae Miltiorrhizae and Cortex Moutan, respectively, were detected and 28 constituents including 14 phenolic acids, 6 diterpenoid quinones, 6 monoterpenoids and 2 other components were identified, while some isomers were distinguished based on the MSn spectra. This method was rapid and reliable for identification of constituents in complex chemical system, ' SHUANGDAN' granules, and the newly fragmentation patterns proposed could be extended to the compounds elucidation with similar framework.
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Authors | Qing He, Xing-Jiang Hu, Yi-Yu Cheng |
Journal | Journal of pharmaceutical and biomedical analysis
(J Pharm Biomed Anal)
Vol. 41
Issue 2
Pg. 485-92
(May 03 2006)
ISSN: 0731-7085 [Print] England |
PMID | 16504448
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cardiovascular Agents
- Cinnamates
- Diterpenes
- Drugs, Chinese Herbal
- Glucosides
- Hydroxybenzoates
- Monoterpenes
- Plant Extracts
- Powders
- Shuangdan
- moutan cortex
- benzoylpaeoniflorin
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Topics |
- Cardiovascular Agents
(chemistry)
- Chromatography, High Pressure Liquid
(methods)
- Cinnamates
(analysis)
- Diterpenes
(analysis)
- Drugs, Chinese Herbal
(chemistry)
- Glucosides
(analysis)
- Hydroxybenzoates
(analysis)
- Molecular Structure
- Molecular Weight
- Monoterpenes
(analysis)
- Paeonia
(chemistry)
- Plant Extracts
- Powders
- Salvia miltiorrhiza
- Spectrometry, Mass, Electrospray Ionization
- Spectrophotometry, Ultraviolet
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