Stimulated-single fiber electromyography monitoring of anti-sense induced changes in experimental autoimmune myasthenia gravis.

Abstract

The neuromuscular weakness associated with myasthenia gravis (MG) can be transiently relieved by pharmacological inhibitors of acetylcholinesterase (AChE). Here, we expand the anticholinesterase repertoire to include 2'-O-methyl-protected antisense oligonucleotides targeted to AChE mRNA (EN101). Using stimulated-single fiber electromyography, we show that EN101 treatment of rats with experimental autoimmune myasthenia gravis (EAMG), improved the mean consecutive difference (MCD) and blocking for 24h. This treatment was more efficient than pyridostigmine and was accompanied by marked improvement in stamina and clinical profile.

• Authors: Neli Boneva, Yasmine Hamra-Amitay, Itzhak Wirguin, Talma Brenner
• Journal: Neuroscience research (Neurosci Res) Vol. 55 Issue 1 Pg. 40-4 (May 2006) ISSN: 0168-0102 [Print] Ireland
• PMID: 16504322 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• EN101
• Oligodeoxyribonucleotides
• Oligonucleotides, Antisense
• RNA, Messenger
• Receptors, Cholinergic
• Acetylcholinesterase

Topics

• Acetylcholinesterase (genetics)
• Animals
• Electric Stimulation (methods)
• Electromyography
• Exercise Test (methods)
• Female
• Monitoring, Physiologic
• Muscles (drug effects, physiopathology, radiation effects)
• Myasthenia Gravis, Autoimmune, Experimental (chemically induced, drug therapy, physiopathology)
• Neuromuscular Junction (drug effects, physiopathology, radiation effects)
• Oligodeoxyribonucleotides
• Oligonucleotides, Antisense (therapeutic use)
• RNA, Messenger (drug effects, genetics, metabolism)
• Rats
• Rats, Inbred Lew
• Receptors, Cholinergic (immunology)
• Time Factors