Abstract | PURPOSE: METHODS:
Laser was used to induce CNV in each eye of 44 rats. For angiography studies, injections of either a mixture of PCNA-Rz 10 microg/microL and 5-FU 1.5 microg/microL, versus the same dose of either drug alone versus a control injection of Hanks' Balanced Salt Solution (HBSS) were performed. We also studied this regimen to evaluate scar size and volume. RESULTS: There was significantly less angiographic leakage for the treated eyes compared to the controls by 3.53 grading points (P = 0.0005); CNV leakage was reduced in the combination group compared to 5-FU alone by 1.75 grading units (P = 0.04) and compared to PCNARz by 2.22 grading units (P = 0.07). The scar size and volume were smaller (diameter 354.6 +/- 174.2 microm vs 477.3 +/- 157.0 microm), (thickness 52.7 +/- 43.0 microm versus 79.6 +/- 46.2 microm) with a reduction in scar volume of 44.8%. CONCLUSIONS: Subretinal injection of PCNA-Rz and 5-FU mixture is more effective as treatment of laser-induced CNV, than either drug alone. The majority of the antiangiogenic effect is a result of 5-FU activity with a contribution by the PCNA ribozyme.
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Authors | Ozcan R Kayikcioglu, Iryna Falkenstein, Linguen Cheng, Igor Kozak, Laurie La Bree, Stanley P Azen, Flossie Wong-Staal, Nicholas F Paoni, William R Freeman |
Journal | Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
(J Ocul Pharmacol Ther)
Vol. 22
Issue 1
Pg. 1-9
(Feb 2006)
ISSN: 1080-7683 [Print] United States |
PMID | 16503769
(Publication Type: Journal Article)
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Chemical References |
- Antimetabolites, Antineoplastic
- Proliferating Cell Nuclear Antigen
- RNA, Catalytic
- hammerhead ribozyme
- Fluorouracil
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Topics |
- Animals
- Antimetabolites, Antineoplastic
(therapeutic use)
- Choroidal Neovascularization
(therapy)
- Combined Modality Therapy
- Disease Models, Animal
- Fluorescein Angiography
- Fluorouracil
(therapeutic use)
- Male
- Proliferating Cell Nuclear Antigen
(genetics)
- RNA, Catalytic
(therapeutic use)
- Rats
- Rats, Long-Evans
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