Protection of the hemopoietic system by Podophyllum hexandrum against gamma radiation-induced damage.

Abstract

A semi-purified extract of low-altitude Podophyllum hexandrum (REC-2001) containing a relatively low content of podophyllotoxin (3.25 %) exhibited potent antioxidant ability in lipid media (at 1000 microg/mLagainst 0.25 kGy) and significant (p < 0.05) hydroxyl ion scavenging potential (78.83 % at 500 microg/mL). In vitro investigations revealed the ability of REC-2001 to significantly (p < 0.05) reduce radiation-induced hemolysis (2 microg/mL; 46.184 %) and nitric oxide scavenging levels (IC (50): 792 +/- 1.25 microg/mL). Protection of the hemopoietic system of Strain 'A' mice administered 20 mg/kg BW REC-2001 30 min prior to lethal irradiation (10 Gy) was recorded and was mediated by free radical scavenging and lowering of lipid oxidation. Further studies investigating the effects of REC-2001 on stem cell modulation are warranted.

• Authors: Ravinder Kumar Sagar, Raman Chawla, Rajesh Arora, Shikha Singh, Bal Krishna, Rakesh Kumar Sharma, Satish Chandra Puri, Pankaj Singh, Raj Kumar, Ashok Kumar Sharma, Surender Singh, Jagdish Prasad, Vinay Gupta, Bilal Ahmed, Kanaya Lal Dhar, Haider Ali Khan, Manju Lata Gupta, Ghulam Nabi Qazi
• Journal: Planta medica (Planta Med) Vol. 72 Issue 2 Pg. 114-20 (Feb 2006) ISSN: 0032-0943 [Print] Germany
• PMID: 16491445 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Drugs, Chinese Herbal
• Radiation-Protective Agents
• Sinopodophyllum hexandrum extract
• Podophyllotoxin

Topics

• Animals
• Antioxidants (chemistry, isolation & purification, pharmacology)
• Berberidaceae
• Drugs, Chinese Herbal (chemistry, isolation & purification, pharmacology)
• Erythrocytes (drug effects, radiation effects)
• Gamma Rays
• Hematopoiesis (drug effects, radiation effects)
• Humans
• Leukocytes (drug effects, radiation effects)
• Mice
• Podophyllotoxin (chemistry, isolation & purification)
• Podophyllum (chemistry)
• Radiation-Protective Agents (chemistry, pharmacology)