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Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats.

Abstract
HLA-B27 transgenic (TG) rats develop spontaneous colitis when colonized with intestinal bacteria, whereas athymic nude (rnu/rnu) HLA-B27 TG rats remain disease free. The present study was designed to determine whether or not HLA-B27 expression on T cells is required for development of colitis after transfer of mesenteric lymph node (MLN) cells into rnu/rnu HLA-B27 recipients. Athymic nontransgenic (non-TG) and HLA-B27 TG recipients received MLN cells from either TG or non-TG rnu/+ heterozygous donor rats that contain T cells. HLA-B27 TG rnu/rnu recipients receiving either non-TG or TG MLN cells developed severe colitis and had higher caecal MPO and IL-1beta levels, and their MLN cells produced more IFN-gamma and less IL-10 after in vitro stimulation with caecal bacterial lysate compared to rnu/rnu non-TG recipients that remained disease free after receiving either TG or non-TG cells. Interestingly, proliferating donor TG T cells were detectable one week after adoptive transfer into rnu/rnu TG recipients but not after transfer into non-TG recipients. T cells from either non-TG or TG donors induce colitis in rnu/rnu TG but not in non-TG rats, suggesting that activation of effector T cells by other cell types that express HLA-B27 is pivotal for the pathogenesis of colitis in this model.
AuthorsF Hoentjen, S L Tonkonogy, B Liu, R B Sartor, J D Taurog, L A Dieleman
JournalClinical and experimental immunology (Clin Exp Immunol) Vol. 143 Issue 3 Pg. 474-83 (Mar 2006) ISSN: 0009-9104 [Print] England
PMID16487247 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • Cell Extracts
  • Cytokines
  • HLA-B27 Antigen
  • Interleukin-1
  • Peroxidase
Topics
  • Adoptive Transfer
  • Animals
  • Animals, Genetically Modified
  • Bacteria (immunology)
  • Cecum (immunology, microbiology)
  • Cell Extracts (immunology)
  • Cell Proliferation
  • Colitis (etiology, immunology, microbiology, pathology)
  • Cytokines (biosynthesis)
  • Disease Models, Animal
  • HLA-B27 Antigen (genetics, metabolism)
  • Interleukin-1 (immunology)
  • Lymphocyte Activation
  • Lymphocyte Transfusion
  • Mesentery
  • Peroxidase (metabolism)
  • Rats
  • Rats, Inbred F344
  • Rats, Nude

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