Abstract |
HLA-B27 transgenic (TG) rats develop spontaneous colitis when colonized with intestinal bacteria, whereas athymic nude (rnu/rnu) HLA-B27 TG rats remain disease free. The present study was designed to determine whether or not HLA-B27 expression on T cells is required for development of colitis after transfer of mesenteric lymph node (MLN) cells into rnu/rnu HLA-B27 recipients. Athymic nontransgenic (non-TG) and HLA-B27 TG recipients received MLN cells from either TG or non-TG rnu/+ heterozygous donor rats that contain T cells. HLA-B27 TG rnu/rnu recipients receiving either non-TG or TG MLN cells developed severe colitis and had higher caecal MPO and IL-1beta levels, and their MLN cells produced more IFN-gamma and less IL-10 after in vitro stimulation with caecal bacterial lysate compared to rnu/rnu non-TG recipients that remained disease free after receiving either TG or non-TG cells. Interestingly, proliferating donor TG T cells were detectable one week after adoptive transfer into rnu/rnu TG recipients but not after transfer into non-TG recipients. T cells from either non-TG or TG donors induce colitis in rnu/rnu TG but not in non-TG rats, suggesting that activation of effector T cells by other cell types that express HLA-B27 is pivotal for the pathogenesis of colitis in this model.
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Authors | F Hoentjen, S L Tonkonogy, B Liu, R B Sartor, J D Taurog, L A Dieleman |
Journal | Clinical and experimental immunology
(Clin Exp Immunol)
Vol. 143
Issue 3
Pg. 474-83
(Mar 2006)
ISSN: 0009-9104 [Print] England |
PMID | 16487247
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Extracts
- Cytokines
- HLA-B27 Antigen
- Interleukin-1
- Peroxidase
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Topics |
- Adoptive Transfer
- Animals
- Animals, Genetically Modified
- Bacteria
(immunology)
- Cecum
(immunology, microbiology)
- Cell Extracts
(immunology)
- Cell Proliferation
- Colitis
(etiology, immunology, microbiology, pathology)
- Cytokines
(biosynthesis)
- Disease Models, Animal
- HLA-B27 Antigen
(genetics, metabolism)
- Interleukin-1
(immunology)
- Lymphocyte Activation
- Lymphocyte Transfusion
- Mesentery
- Peroxidase
(metabolism)
- Rats
- Rats, Inbred F344
- Rats, Nude
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