Production of three monoclonal antibodies specifically reactive respectively with the ductal, acinar and islet cells of the human pancreas.

Abstract

Three monoclonal antibodies, designated FP-1, FP-2 and FP-3, were obtained by immunizing a BALB/c mouse with dispersed human fetal pancreatic cells and using hybridoma technology. FP-1, FP-2 and FP-3 reacted specifically, respectively, with the ductal epithelial, acinar and islet cells, of the fetal pancreas as well as with adult human pancreatic tissue, suggesting a use in the discrimination of pancreatic cell types. Upon screening various tumor tissues, FP-1 was found to react with adenocarcinomas of the pancreas (16/23), stomach (7/8), colon (4/6) and gall bladder (2/2) as well as with the three malignant cell lines, PANC-1 (pancreas), HT-29 (colon) and LoVo (colon). In contrast to FP-1, FP-2 and FP-3 reacted with only 5% (2/39) and 0% (0/39), respectively, of these adenocarcinomas. Although FP-3 did not react with any of these adenocarcinomas, it did react with various APUDoma cells such as islet cell tumors of the pancreas (3/5), pheochromocytomas (2/2), medullary thyroid carcinomas (2/3) and gastrointestinal carcinoids (1/3). FP-3 thus appears to be the first monoclonal antibody with extremely high endocrine cell specificity.

• Authors: K Shibata, T Kobayashi, N Matsuura, T Shimano, T Mori
• Journal: Japanese journal of clinical oncology (Jpn J Clin Oncol) Vol. 21 Issue 1 Pg. 13-21 (Feb 1991) ISSN: 0368-2811 [Print] England
• PMID: 1648632 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• oncofetal antigens

Topics

• Animals
• Antibodies, Monoclonal (biosynthesis)
• Antibody Specificity
• Antigens, Neoplasm (immunology)
• Carcinoma (immunology)
• Carcinoma, Intraductal, Noninfiltrating (immunology)
• Clone Cells
• Humans
• Hybridomas
• Immunoenzyme Techniques
• Immunohistochemistry
• Mice
• Mice, Inbred BALB C
• Pancreas (embryology, immunology)
• Pancreatic Neoplasms (immunology)