Anti-idiotypic antibodies to feline leukemia virus: an approach for retroviral immunization strategies.

The present study describes an approach to the development and use of anti-idiotypic antibodies as a possible immunization strategy to prevent retroviral infection. The rationale for using anti-idiotypes (anti-Ids) to try to elicit an antigenic-specific immune response is examined, and the production and characterization of polyclonal and monoclonal anti-Ids are described. Several techniques were used to determine antigenic mimicry and anti-Id subtypes. The potential use of anti-Ids in feline leukemia virus (FeLV) receptor studies and vaccine trials in vivo were investigated. Results from these studies suggest that the anti-Id strategy is feasible for the FeLV model. Polyclonal Ab2 reagents were developed that blocked virus-receptor binding and thus inhibited viral infection in vitro and induced humoral immune responses in 6- to 8-week old kittens characterized by production of Ab3 with the ability to bind the original FeLV envelope protein gp70 as assessed by Western blot analysis.
AuthorsL Tavares, C Roneker, L Postie, M Fevereiro, F de Noronha
JournalViral immunology (Viral Immunol) Vol. 4 Issue 1 Pg. 5-16 ( 1991) ISSN: 0882-8245 [Print] UNITED STATES
PMID1648360 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Anti-Idiotypic
  • Antibodies, Viral
  • Immunoglobulin G
  • Viral Envelope Proteins
  • Viral Vaccines
  • Animals
  • Antibodies, Anti-Idiotypic (biosynthesis, immunology)
  • Antibodies, Viral (analysis, immunology)
  • Blotting, Western
  • Cats
  • Chromatography, Affinity
  • Enzyme-Linked Immunosorbent Assay
  • Feline Acquired Immunodeficiency Syndrome (immunology, prevention & control)
  • Goats
  • Immunoglobulin G (immunology)
  • Leukemia Virus, Feline (immunology)
  • Leukemia, Experimental (immunology, prevention & control)
  • Mice
  • Mice, Inbred BALB C
  • Viral Envelope Proteins (immunology)
  • Viral Vaccines

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