Alzheimer's amyloid beta-peptide (1-42) induces cell death in human neuroblastoma via bax/bcl-2 ratio increase: an intriguing role for methionine 35.

Abstract

The beta amyloid (Abeta), the major protein component of brain senile plaques in Alzheimer's disease, is known to be directly responsible for the production of free radicals toxic to brain tissue and the redox state of Met-35 residue seems to play a particular and critical role in peptide's neurotoxic actions. In this study, we investigated, in human neuroblastoma cells (IMR-32), the relationship between the oxidative state of methionine, and both neurotoxic and pro-apoptotic actions induced by Abeta-peptide, comparing the effects of native peptide, in which the Met-35 is present in the reduced state, with those of a modified peptide with oxidized Met-35 (Abeta(1-42)(35Met-ox)), as well as an Abeta-derivative with Met-35 substituted with norleucine (Abeta(1-42)(35Nle)). The obtained results show that Abeta induces a time-dependent decrease in cell viability; Abeta(1-42)(35Met-ox) was significantly less potent, though inducing a remarkable decrease in cell viability compared to control. On the contrary, no toxic effects were observed after treatment with Abeta(1-42)(35Nle). Abeta-peptide as well as the amyloid modified peptide with oxidized Met-35 induced the pro-apoptotic gene bax over-expression after 24 h, whereas Abeta(1-42)(35Nle) had no effect. Conversely, bcl-2, an anti-apoptotic gene, became highly down-regulated by Abeta peptide treatment, in contrast to that evidenced by the Abeta(1-42)(35Met-ox) peptide. Finally, Abeta caused an increase in caspase-3 activity to be higher with respect to that shown by Abeta(1-42)(35Met-ox) while Abeta(1-42)(35Nle) had no effect. These results support the hypothesis that Abeta-induced neurotoxicity occurs via bax over-expression, bcl-2 down-regulation, and caspase-3 activation, first indicating that methionine 35 redox state may alter this cell death pathway.

• Authors: M E Clementi, M Pezzotti, F Orsini, B Sampaolese, D Mezzogori, C Grassi, B Giardina, F Misiti
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 342 Issue 1 Pg. 206-13 (Mar 31 2006) ISSN: 0006-291X [Print] United States
• PMID: 16472763 (Publication Type: Journal Article)

Chemical References

• Amyloid beta-Peptides
• Peptide Fragments
• Proto-Oncogene Proteins c-bcl-2
• amyloid beta-protein (1-42)
• bcl-2-Associated X Protein
• Methionine
• CASP3 protein, human
• Caspase 3
• Caspases

Topics

• Alzheimer Disease (metabolism)
• Amyloid beta-Peptides (chemistry, pharmacology)
• Caspase 3
• Caspases (metabolism)
• Cell Death (drug effects)
• Gene Expression Regulation (drug effects)
• Humans
• Methionine (metabolism)
• Mitochondria (drug effects)
• Neuroblastoma (genetics, metabolism, pathology)
• Peptide Fragments (chemistry, pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (genetics)
• bcl-2-Associated X Protein (genetics)