Studies on in vitro expression and in vivo immunogenicity of a recombinant MVA HIV vaccine.

Here we conduct dose-response studies for in vitro expression and in vivo immunogenicity for a recombinant modified vaccinia Ankara (MVA) vaccine that expresses HIV Gag, Pol, and Env proteins. The dose-response studies for in vitro expression used fluorescent-activated cell sorting to score Gag- and Env-expressing cells and showed good increases for antigen expression with increasing MVA dose. In these studies, a 1000-fold increase in the dose of MVA resulted in a 300-fold increase in the frequency of antigen-expressing cells. In contrast, dose-response studies for in vivo immunogenicity showed <10-fold increases in elicited T cells and Ab for 100-1000-fold increases in the dose of inoculated MVA. These studies used intracellular cytokine assays to enumerate responding CD8 and CD4 T cells and an ELISA to score anti-Env Ab. The shallow dose-response curves for immunogenicity were observed post priming as well as post boosting of an MVA or a DNA prime.
AuthorsJinyan Liu, Linda S Wyatt, Rama Rao Amara, Bernard Moss, Harriet L Robinson
JournalVaccine (Vaccine) Vol. 24 Issue 16 Pg. 3332-9 (Apr 12 2006) ISSN: 0264-410X [Print] Netherlands
PMID16472543 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • AIDS Vaccines
  • Cytokines
  • Gene Products, env
  • Gene Products, gag
  • Gene Products, pol
  • HIV Antibodies
  • Vaccines, DNA
  • Vaccines, Synthetic
  • Viral Vaccines
  • AIDS Vaccines (administration & dosage, genetics, immunology)
  • Animals
  • CD4-Positive T-Lymphocytes (immunology)
  • CD8-Positive T-Lymphocytes (immunology)
  • Cell Line
  • Cytokines (biosynthesis)
  • Dose-Response Relationship, Immunologic
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gene Expression
  • Gene Products, env (genetics, immunology)
  • Gene Products, gag (genetics, immunology)
  • Gene Products, pol (genetics, immunology)
  • HIV Antibodies (blood)
  • Humans
  • Lymphocyte Subsets (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Vaccines, DNA (administration & dosage, genetics, immunology)
  • Vaccines, Synthetic (administration & dosage, genetics, immunology)
  • Vaccinia virus (genetics, growth & development, immunology)
  • Viral Vaccines (administration & dosage, genetics, immunology)

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