Studies on in vitro expression and in vivo immunogenicity of a recombinant MVA HIV vaccine.

Abstract

Here we conduct dose-response studies for in vitro expression and in vivo immunogenicity for a recombinant modified vaccinia Ankara (MVA) vaccine that expresses HIV Gag, Pol, and Env proteins. The dose-response studies for in vitro expression used fluorescent-activated cell sorting to score Gag- and Env-expressing cells and showed good increases for antigen expression with increasing MVA dose. In these studies, a 1000-fold increase in the dose of MVA resulted in a 300-fold increase in the frequency of antigen-expressing cells. In contrast, dose-response studies for in vivo immunogenicity showed <10-fold increases in elicited T cells and Ab for 100-1000-fold increases in the dose of inoculated MVA. These studies used intracellular cytokine assays to enumerate responding CD8 and CD4 T cells and an ELISA to score anti-Env Ab. The shallow dose-response curves for immunogenicity were observed post priming as well as post boosting of an MVA or a DNA prime.

• Authors: Jinyan Liu, Linda S Wyatt, Rama Rao Amara, Bernard Moss, Harriet L Robinson
• Journal: Vaccine (Vaccine) Vol. 24 Issue 16 Pg. 3332-9 (Apr 12 2006) ISSN: 0264-410X [Print] Netherlands
• PMID: 16472543 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• AIDS Vaccines
• Cytokines
• Gene Products, env
• Gene Products, gag
• Gene Products, pol
• HIV Antibodies
• Vaccines, DNA
• Vaccines, Synthetic
• Viral Vaccines

Topics

• AIDS Vaccines (administration & dosage, genetics, immunology)
• Animals
• CD4-Positive T-Lymphocytes (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Cell Line
• Cytokines (biosynthesis)
• Dose-Response Relationship, Immunologic
• Enzyme-Linked Immunosorbent Assay
• Female
• Gene Expression
• Gene Products, env (genetics, immunology)
• Gene Products, gag (genetics, immunology)
• Gene Products, pol (genetics, immunology)
• HIV Antibodies (blood)
• Humans
• Lymphocyte Subsets (immunology)
• Mice
• Mice, Inbred BALB C
• Vaccines, DNA (administration & dosage, genetics, immunology)
• Vaccines, Synthetic (administration & dosage, genetics, immunology)
• Vaccinia virus (genetics, growth & development, immunology)
• Viral Vaccines (administration & dosage, genetics, immunology)