HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Studies on in vitro expression and in vivo immunogenicity of a recombinant MVA HIV vaccine.

Abstract
Here we conduct dose-response studies for in vitro expression and in vivo immunogenicity for a recombinant modified vaccinia Ankara (MVA) vaccine that expresses HIV Gag, Pol, and Env proteins. The dose-response studies for in vitro expression used fluorescent-activated cell sorting to score Gag- and Env-expressing cells and showed good increases for antigen expression with increasing MVA dose. In these studies, a 1000-fold increase in the dose of MVA resulted in a 300-fold increase in the frequency of antigen-expressing cells. In contrast, dose-response studies for in vivo immunogenicity showed <10-fold increases in elicited T cells and Ab for 100-1000-fold increases in the dose of inoculated MVA. These studies used intracellular cytokine assays to enumerate responding CD8 and CD4 T cells and an ELISA to score anti-Env Ab. The shallow dose-response curves for immunogenicity were observed post priming as well as post boosting of an MVA or a DNA prime.
AuthorsJinyan Liu, Linda S Wyatt, Rama Rao Amara, Bernard Moss, Harriet L Robinson
JournalVaccine (Vaccine) Vol. 24 Issue 16 Pg. 3332-9 (Apr 12 2006) ISSN: 0264-410X [Print] Netherlands
PMID16472543 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • AIDS Vaccines
  • Cytokines
  • Gene Products, env
  • Gene Products, gag
  • Gene Products, pol
  • HIV Antibodies
  • Vaccines, DNA
  • Vaccines, Synthetic
  • Viral Vaccines
Topics
  • AIDS Vaccines (administration & dosage, genetics, immunology)
  • Animals
  • CD4-Positive T-Lymphocytes (immunology)
  • CD8-Positive T-Lymphocytes (immunology)
  • Cell Line
  • Cytokines (biosynthesis)
  • Dose-Response Relationship, Immunologic
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gene Expression
  • Gene Products, env (genetics, immunology)
  • Gene Products, gag (genetics, immunology)
  • Gene Products, pol (genetics, immunology)
  • HIV Antibodies (blood)
  • Humans
  • Lymphocyte Subsets (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Vaccines, DNA (administration & dosage, genetics, immunology)
  • Vaccines, Synthetic (administration & dosage, genetics, immunology)
  • Vaccinia virus (genetics, growth & development, immunology)
  • Viral Vaccines (administration & dosage, genetics, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: