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Model for studying virus attachment. II. Binding of biotinylated human T cell leukemia virus type I to human blood mononuclear cells potential targets for human T cell leukemia virus type I infection.

Abstract
Purified human T cell leukemia virus type I (HTLV-I) was biotinylated and used to study its attachment to human PBMC. The use of biotinylated HTLV-I (biot-HTLV-I) in conjunction with mouse mAb specific for selected cell-surface molecules and flow cytometric analysis allowed us to positively identify virus-binding cells among a heterogeneous blood mononuclear cell population. Biot-HTLV-I efficiently bound not only to T cells, but also to B cells and monocytes. Preincubation of monocytes with excess of unlabeled HTLV-I significantly reduced the attachment of biot-HTLV-I. HTLV-I not only bound to, but also infected, B cells, as suggested by: i) in situ hybridization of a 35S-labeled full length HTLV-I DNA probe with EBV-transformed B cells, previously cocultured with HTLV-I-producing (G11MJ) T cells, and ii) hybridization of the same nick-translated 32P-labeled DNA probe with blotted DNA from similar HTLV-I-infected EBV-transformed B cells. HTLV-I infection did not affect the ability of B cells to secrete IgG. These findings suggest that HTLV-I cannot only infect cells of the T lineage, but can also infect B cells.
AuthorsS Dhawan, H Z Streicher, L M Wahl, N Miller, A T Louie, I S Goldfarb, W L Jackson, P Casali, A L Notkins
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 147 Issue 1 Pg. 102-8 (Jul 01 1991) ISSN: 0022-1767 [Print] United States
PMID1646840 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Receptors, Virus
  • Biotin
Topics
  • Antibody Formation
  • Antibody-Producing Cells (immunology, metabolism)
  • B-Lymphocytes (metabolism, microbiology)
  • Binding, Competitive
  • Biotin
  • Cell Transformation, Viral
  • Flow Cytometry
  • HTLV-I Infections (microbiology)
  • Herpesvirus 4, Human
  • Human T-lymphotropic virus 1 (metabolism)
  • Humans
  • In Vitro Techniques
  • Leukocytes, Mononuclear (metabolism, microbiology)
  • Monocytes (metabolism, microbiology)
  • Receptors, Virus (metabolism)
  • T-Lymphocytes (metabolism, microbiology)

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