Abstract |
Purified human T cell leukemia virus type I (HTLV-I) was biotinylated and used to study its attachment to human PBMC. The use of biotinylated HTLV-I (biot-HTLV-I) in conjunction with mouse mAb specific for selected cell-surface molecules and flow cytometric analysis allowed us to positively identify virus-binding cells among a heterogeneous blood mononuclear cell population. Biot-HTLV-I efficiently bound not only to T cells, but also to B cells and monocytes. Preincubation of monocytes with excess of unlabeled HTLV-I significantly reduced the attachment of biot-HTLV-I. HTLV-I not only bound to, but also infected, B cells, as suggested by: i) in situ hybridization of a 35S-labeled full length HTLV-I DNA probe with EBV-transformed B cells, previously cocultured with HTLV-I-producing (G11MJ) T cells, and ii) hybridization of the same nick-translated 32P-labeled DNA probe with blotted DNA from similar HTLV-I-infected EBV-transformed B cells. HTLV-I infection did not affect the ability of B cells to secrete IgG. These findings suggest that HTLV-I cannot only infect cells of the T lineage, but can also infect B cells.
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Authors | S Dhawan, H Z Streicher, L M Wahl, N Miller, A T Louie, I S Goldfarb, W L Jackson, P Casali, A L Notkins |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 147
Issue 1
Pg. 102-8
(Jul 01 1991)
ISSN: 0022-1767 [Print] United States |
PMID | 1646840
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Antibody Formation
- Antibody-Producing Cells
(immunology, metabolism)
- B-Lymphocytes
(metabolism, microbiology)
- Binding, Competitive
- Biotin
- Cell Transformation, Viral
- Flow Cytometry
- HTLV-I Infections
(microbiology)
- Herpesvirus 4, Human
- Human T-lymphotropic virus 1
(metabolism)
- Humans
- In Vitro Techniques
- Leukocytes, Mononuclear
(metabolism, microbiology)
- Monocytes
(metabolism, microbiology)
- Receptors, Virus
(metabolism)
- T-Lymphocytes
(metabolism, microbiology)
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