| Abstract | C/EBP beta (CCAAT/enhancer binding protein beta) is a transcriptional factor that belongs to the basic region-leucine zipper class DNA-binding proteins and plays a role in cell differentiation and inflammatory reactions. Although high tissue levels of inflammatory cytokines, such as interleukin (IL)-6, IL-8 and transforming growth factor-beta, have been observed in glioma patients, the mechanisms underlying this phenomenon remain to be elucidated. C/EBP beta induces a variety of cytokines and thus may play a role in the pathogenesis of glioma. In this study, we investigated the relationship between C/EBP beta expression, tumor histology, and prognosis in glioma. The expression of C/EBP beta mRNA was examined with quantitative real-time PCR and protein expression was examined with immunohistochemical techniques in 47 glioma tissue samples. Expression of C/EBP beta mRNA and protein was markedly increased in high grade glioma compared with low grade glioma. Patients whose expression of C/EBP beta mRNA and protein in tumor tissue was lower survived longer than those whose expressions were higher. In vitro, C/EBP beta siRNA inhibited glioma cell proliferation and invasion. Moreover, IL-8 production by glioma cells was inhibited by C/EBP beta siRNA transfection. These data suggest that increased expression of C/EBP beta may contribute to the promotion of tumor invasiveness and progression. The data imply that the comparison of C/EBP beta expression could be a prognostic marker for patients with glioma. |
| Authors | Jumpei Homma, Ryuya Yamanaka, Naoki Yajima, Naoto Tsuchiya, Nobuyuki Genkai, Masakazu Sano, Ryuichi Tanaka
(Affiliation: Department of Neurosurgery, Brain Research Institute, Niigata University, Niigata 951-8585, Japan.)
|
| Journal | Oncology reports
(Oncol Rep)
Vol. 15
Issue 3
Pg. 595-601
(Mar 2006)
ISSN: 1021-335X Greece |
| PMID | 16465418
(Publication Type: Journal Article)
|
| Chemical References |
- CCAAT-Enhancer-Binding Protein-beta
- Interleukin-8
- RNA, Messenger
- RNA, Small Interfering
|
| Topics |
- Adolescent
- Adult
- Aged
- Astrocytoma
(genetics, metabolism, pathology)
- Brain Neoplasms
(genetics, metabolism, pathology)
- CCAAT-Enhancer-Binding Protein-beta
(analysis, genetics)
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Child
- Female
- Gene Expression
- Glioblastoma
(genetics, metabolism, pathology)
- Glioma
(genetics, metabolism, pathology)
- Humans
- Immunohistochemistry
- Interleukin-8
(secretion)
- Male
- Middle Aged
- Prognosis
- RNA Interference
(physiology)
- RNA, Messenger
(genetics, metabolism)
- RNA, Small Interfering
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Survival Analysis
- Transfection
|
