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Use of engineered peripheral nerve autografts for spinal cord repair.

Abstract
We developed a clinically compatible protocol for the production of engineered tissue for grafting into the injured spinal cord. We used autologous tissue derived from pre-ligated peripheral nerves, which avoids supply, immunocompatibility and ethical hinderances, combined with non-viral transfection, which is a versatile and non-immunogenic gene transfer method. In-vitro transfection of glial cells or primary tissue from pre-ligated rat peripheral nerve with the neurotrophic gene brain-derived neurotrophic factor significantly enhanced its expression, when quantified or labelled by immunofluorescence. Engineered tissue expressed brain-derived neurotrophic factor after being grafted into the spinal cord of rats that had received spinal contusion injury 3 weeks before. Anatomical and functional assays of repair, conducted on a small cohort, showed that the treatment may promote axonal regeneration and improve motor performance.
AuthorsRogan B Tinsley, Shu-Hua Zhang, Shi-Qing Feng, Robert A Rush, Ian A Ferguson
JournalNeuroreport (Neuroreport) Vol. 17 Issue 3 Pg. 261-5 (Feb 27 2006) ISSN: 0959-4965 [Print] England
PMID16462594 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Brain-Derived Neurotrophic Factor
  • Receptors, Growth Factor
  • Polyethyleneimine
  • beta-Galactosidase
Topics
  • Analysis of Variance
  • Animals
  • Brain-Derived Neurotrophic Factor (biosynthesis, genetics, therapeutic use)
  • Cells, Cultured
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Female
  • Genetic Therapy (methods)
  • Immunohistochemistry (methods)
  • Polyethyleneimine (pharmacology)
  • Rats
  • Receptors, Growth Factor (metabolism)
  • Spinal Cord Injuries (therapy)
  • Sural Nerve (physiology, transplantation)
  • Transfection (methods)
  • Transplantation, Autologous (methods)
  • beta-Galactosidase (metabolism)

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