The repressive effect of green tea ingredients on amyloid precursor protein (APP) expression in oral carcinoma cells in vitro and in vivo.

Abstract

In a hamster model of N-methyl-N-benzylnitrosamine (MBN)-induced oral carcinogenesis, the incidence of buccal pouch (HBP) carcinomas in MBN-treated hamsters (17.8+/-7.5) was significantly higher than MBN-treated hamsters given tea (10.8+/-3.9) (P<0.05). Amyloid precursor protein (APP) expression was also significantly increased in MBN-induced HBP carcinomas but was significantly reduced by tea intake (P<0.0001). Furthermore, APP expression and secretion by OECM-1 oral squamous cell carcinoma cells was inhibited by a major polyphenolic ingredient of green tea, (-)-epigallocatechin gallate, in a dose-dependent manner. Thus, APP might promote oral carcinogenesis, whereas green tea ingredients might diminish it by down-regulating APP.

• Authors: Shun-Yao Ko, Kuo-Wei Chang, Shu-Chun Lin, Hui-Chuan Hsu, Tsung-Yun Liu
• Journal: Cancer letters (Cancer Lett) Vol. 245 Issue 1-2 Pg. 81-9 (Jan 08 2007) ISSN: 0304-3835 [Print] Ireland
• PMID: 16458426 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amyloid beta-Protein Precursor
• Plant Preparations
• RNA, Messenger
• Tea
• Catechin
• epigallocatechin gallate

Topics

• Amyloid beta-Protein Precursor (analysis, genetics)
• Animals
• Blotting, Western
• Carcinoma, Squamous Cell (drug therapy, genetics, pathology)
• Catechin (analogs & derivatives, pharmacology)
• Cell Line, Tumor
• Cricetinae
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Immunohistochemistry
• Male
• Mesocricetus
• Mouth Mucosa (drug effects, metabolism, pathology)
• Mouth Neoplasms (drug therapy, genetics, pathology)
• Phytotherapy
• Plant Preparations (chemistry, pharmacology)
• RNA, Messenger (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Tea (chemistry)