Abstract | INTRODUCTION:
Rapamycin, an inhibitor of the serine/threonine kinase target of rapamycin, induces G1 arrest and/or apoptosis. Although rapamycin and its analogues are attractive candidates for cancer therapy, their sensitivities with respect to growth inhibition differ markedly among various cancer cells. Using human breast carcinoma cell line MCF-7 as an experimental model system, we examined the growth-inhibitory effects of combinations of various agents and rapamycin to find the agent that most potently enhances the growth-inhibitory effect of rapamycin. METHOD: We evaluated the growth-inhibitory effect of rapamycin plus various agents, including cotylenin A (a novel inducer of differentiation of myeloid leukaemia cells) to MCF-7 cells, using either MTT assay or trypan blue dye exclusion test. The cell cycle was analyzed using propidium iodide-stained nuclei. Expressions of several genes in MCF-7 cells with rapamycin plus cotylenin A were studied using cDNA microarray analysis and RT-PCR. The in vitro results of MCF-7 cells treated with rapamycin plus cotylenin A were further confirmed in vivo in a mouse xenograft model. RESULTS: CONCLUSION:
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Authors | Takashi Kasukabe, Junko Okabe-Kado, Nobuo Kato, Takeshi Sassa, Yoshio Honma |
Journal | Breast cancer research : BCR
(Breast Cancer Res)
Vol. 7
Issue 6
Pg. R1097-110
( 2005)
ISSN: 1465-542X [Electronic] England |
PMID | 16457690
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibiotics, Antineoplastic
- Diterpenes
- cotylenin A
- Sirolimus
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Topics |
- Animals
- Antibiotics, Antineoplastic
(pharmacology)
- Breast Neoplasms
(pathology)
- Carcinoma
(pathology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Diterpenes
(pharmacology)
- Drug Interactions
- Female
- Gene Expression Regulation, Neoplastic
(drug effects)
- Genes, Neoplasm
- Humans
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Sirolimus
(pharmacology)
- Transplantation, Heterologous
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